000806788 000__ 05647cam\a2200529Ii\4500 000806788 001__ 806788 000806788 005__ 20230306143836.0 000806788 006__ m\\\\\o\\d\\\\\\\\ 000806788 007__ cr\cn\nnnunnun 000806788 008__ 170217s2017\\\\sz\a\\\\ob\\\\001\0\eng\d 000806788 019__ $$a973314515$$a973378756$$a973532064$$a973774632$$a973809199$$a973913600$$a981774198$$a1005784895$$a1011792309 000806788 020__ $$a9783319482606$$q(electronic book) 000806788 020__ $$a3319482602$$q(electronic book) 000806788 020__ $$z9783319482590 000806788 020__ $$z3319482599 000806788 0247_ $$a10.1007/978-3-319-48260-6$$2doi 000806788 035__ $$aSP(OCoLC)ocn972900308 000806788 035__ $$aSP(OCoLC)972900308$$z(OCoLC)973314515$$z(OCoLC)973378756$$z(OCoLC)973532064$$z(OCoLC)973774632$$z(OCoLC)973809199$$z(OCoLC)973913600$$z(OCoLC)981774198$$z(OCoLC)1005784895$$z(OCoLC)1011792309 000806788 040__ $$aN$T$$beng$$erda$$epn$$cN$T$$dEBLCP$$dN$T$$dIDEBK$$dGW5XE$$dYDX$$dOCLCF$$dDKU$$dCOO$$dAZU$$dUPM$$dMERER$$dZ5A$$dOCLCQ$$dMERUC$$dVT2$$dJG0$$dUAB 000806788 049__ $$aISEA 000806788 050_4 $$aQC477 000806788 08204 $$a535/.352$$223 000806788 24500 $$aReviews in fluorescence 2016 /$$cChris D. Geddes, editor. 000806788 264_1 $$aCham, Switzerland :$$bSpringer,$$c[2017] 000806788 300__ $$a1 online resource :$$billustrations. 000806788 336__ $$atext$$btxt$$2rdacontent 000806788 337__ $$acomputer$$bc$$2rdamedia 000806788 338__ $$aonline resource$$bcr$$2rdacarrier 000806788 347__ $$atext file$$bPDF$$2rda 000806788 4901_ $$aReviews in fluorescence ;$$vvolume 9 000806788 504__ $$aIncludes bibliographical references and index. 000806788 5050_ $$aPreface; Contents; 1 Fluorescence Made Easier: Fluorescence Techniques for the Novice. Episode 1: The Basics; 1.1 Basic Concepts, with a Minimum of Annoying Physics: The Golden Rules; 1.1.1 Golden Rule 1: The Fluorescence Is Always at Longer Wavelengths than the Excitation; 1.1.2 Golden Rule 2: The Shape of the Emission Spectrum Does Not Change as the Excitation Wavelength Is Varied; 1.1.2.1 Quantum Yield; 1.1.2.2 Measuring Fluorescence Intensities and Spectra; 1.1.3 Golden Rule 3: Ordinarily, the Excitation Spectrum Is the Same Shape as the Absorption Spectrum; Glossary. 000806788 5058_ $$a2 Fluorescence Made Easier: Fluorescence Techniques for the Novice. Episode 2: Using Fluorescence Anisotropy or Polarization to View Intermolecular Associations2.1 What's Behind Fluorescence Anisotropy?; 2.2 To Understand Fluorescence Anisotropy or Polarization, You Need to Know One Simple Equation; 2.3 To Measure Fluorescence Anisotropy or Polarization, You Need to Think About Light; References; 3 Time-Gated FRET Detection for Multiplexed Biosensing; 3.1 Introduction; 3.2 Theory of Time-Gated (TG) FRET; 3.2.1 Time-Gated vs. Time-Resolved Luminescence. 000806788 5058_ $$a3.2.2 TG FRET (Förster Resonance Energy Transfer)3.2.3 Multiplexed TG FRET; 3.3 Advantages of TG FRET; 3.3.1 Single Analyte Detection; 3.3.2 Multiplexed Detection; 3.4 Spectroscopy-Based TG FRET Biosensing; 3.4.1 Equipment and Detection Formats; 3.4.2 Single Analyte Biosensing Applications; 3.4.3 Multiplexed Biosensing Applications; 3.5 Microscopy-Based TG FRET Biosensing; 3.5.1 Equipment and Detection Formats; 3.5.2 TG Luminescence Biosensing; 3.5.3 TG FRET Biosensing; 3.6 Conclusions and Future Perspectives; References. 000806788 5058_ $$a4 Pharmacologically Active Plant Flavonols as Proton Transfer Based Multiparametric Fluorescence Probes Targeting Biomolecules: Perspectives and Prospects4.1 Introduction: Background and Historical Perspectives; 4.1.1 Fluorescence as an Exquisitely Sensitive Approach for Exploring Biomolecular Interactions: A Brief Overview; 4.1.2 Plant Flavonoids: Natural Distribution, Chemical Classification, Therapeutic Importance, and Intrinsic Fluorescence Behavior. 000806788 5058_ $$a4.1.3 Excited-State Intramolecular Proton Transfer (ESIPT) and `Two Color' Fluorescence of Flavonols: Historical Perspective and Sensitivity to Environment4.2 Applications of ESIPT and `Two Color' Fluorescence of Therapeutically Active Flavonols with Representative Bio-relevant Targets: Scope of the Present Survey and Fluorescence Methodologies; 4.3 Protein-Flavonol Interactions; 4.3.1 Background; 4.3.2 Serum Albumin-Flavonol Interactions; 4.3.3 Hemoglobin-Flavonol Interaction; 4.4 DNA-Flavonol Interactions; 4.4.1 Background; 4.4.2 Duplex DNA-Flavonol Interactions. 000806788 506__ $$aAccess limited to authorized users. 000806788 520__ $$aReviews in Fluorescence 2016, the tenth volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the yearℓ́ℓs progress in fluorescence and its applications, with authoritative reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. Reviews in Fluorescence offers an essential reference material for any research lab or company working in the fluorescence field and related areas. All academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in the continuously emerging field of fluorescence will find it an invaluable resource. 000806788 588__ $$aOnline resource; title from PDF title page (viewed February 21, 2017). 000806788 650_0 $$aFluorescence. 000806788 7001_ $$aGeddes, Chris D.,$$eeditor. 000806788 77608 $$iPrint version:$$tReviews in fluorescence 2016.$$dCham, Switzerland : Springer, [2017]$$z3319482599$$z9783319482590$$w(OCoLC)959034246 000806788 830_0 $$aReviews in fluorescence ;$$vv. 9. 000806788 852__ $$bebk 000806788 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-319-48260-6$$zOnline Access$$91397441.1 000806788 909CO $$ooai:library.usi.edu:806788$$pGLOBAL_SET 000806788 980__ $$aEBOOK 000806788 980__ $$aBIB 000806788 982__ $$aEbook 000806788 983__ $$aOnline 000806788 994__ $$a92$$bISE