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Title
Enzyme-linked immunosorbent assay (ELISA) : from A to Z / Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa.
ISBN
9789811067662 (electronic book)
981106766X (electronic book)
9789811067655
9811067651
Published
Singapore : Springer, [2018]
Copyright
©2018
Language
English
Description
1 online resource.
Item Number
10.1007/978-981-10-6766-2 doi
Call Number
QP519.9.E48
Dewey Decimal Classification
616.07/56
Summary
This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.#xE000.
Bibliography, etc. Note
Includes bibliographical references.
Access Note
Access limited to authorized users.
Digital File Characteristics
text file PDF
Source of Description
Online resource; title from PDF title page (viewed January 9, 2018).
Series
SpringerBriefs in applied sciences and technology. Forensic and medical bioinformatics.
1. Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA
2. General overview on applications of ELISA
3. Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities
4. Evaluation of the detection results obtained from ELISA
5. Advantages, disadvantages and modifications of conventional ELISA
6.Common questions and answers about ELISA.
