Biosensors based on sandwich assays / Fan Xia, Xiaojin Zhang, Xiaoding Lou, Quan Yuan, editors.
Xia, Fan, editor.; Zhang, Xiaojin, editor.; Lou, Xiaoding, editor.; Yuan, Quan, editor.
2018
R857.B54
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Title
Biosensors based on sandwich assays / Fan Xia, Xiaojin Zhang, Xiaoding Lou, Quan Yuan, editors.
ISBN
9789811078354 (electronic book)
9811078351 (electronic book)
9789811078347
9811078343
DOI
https://doi.org/10.1007/978-981-10-7835-4
Published
Singapore : Springer, [2018]
Copyright
©2018
Language
English
Description
1 online resource
Item Number
10.1007/978-981-10-7835-4 doi
Call Number
R857.B54
Dewey Decimal Classification
610.28
Summary
This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.
Bibliography, etc. Note
Includes bibliographical references.
Access Note
Access limited to authorized users.
Digital File Characteristics
text file PDF
Source of Description
Vendor-supplied metadata.
Added Author
Xia, Fan, editor.
Zhang, Xiaojin, editor.
Lou, Xiaoding, editor.
Yuan, Quan, editor.
Available in Other Form
Biosensors based on sandwich assays.
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Intro; Contents; Editors and Contributors; 1 Introduction; Abstract; 1.1 What Are the Sandwich Assays?; 1.2 Categories of the Sandwich Assays; 1.3 Sandwich Assays for Protein Detection; 1.4 Sandwich Assays for Nucleic Acid Detection; 1.5 Sandwich Assays for Small Molecule and Ion Detection; 1.6 Sandwich Assays for Cell Detection; 1.7 Supersandwich Assays; 1.8 Conclusion; References; 2 Colorimetric Sandwich Assays for Protein Detection; Abstract; 2.1 Introduction; 2.2 Colorimetric Sandwich Assays Based on the Traditional Enzymes.

2.2.1 Alkaline Phosphatase (ALP) for Colorimetric Sandwich Assays2.2.1.1 Horseradish Peroxidase (HRP) for Colorimetric Sandwich Assays; 2.2.2 Open Sandwich Immunoassays (OS-IA); 2.2.3 Goldâ#x80;#x93;Multienzymeâ#x80;#x93;Nanocarrier-Based Colorimetric Sandwich Assays; 2.3 Colorimetric Assays Based on Biomimetic Nanomaterials; 2.3.1 Fe3O4 Magnetic Nanoparticles (MNPs) Biomimetic Enzymes; 2.3.2 Au@Pt Nanostructure Biomimetic Enzymes; 2.4 Conclusion; References; 3 Fluorescence Sandwich Assays for Protein Detection; Abstract; 3.1 Introduction; 3.2 Design Principles of Fluorescence Sandwich Assays.

3.3 Antibodies as Recognition Probe or Signal Probe3.4 Aptamers as Recognition Probe or Signal Probe; 3.5 Traditional Organic Dyes as Fluorescence Signal Reporter; 3.6 Nanomaterials as Fluorescence Signal Reporter; 3.7 Conjugated Polymers as Fluorescence Signaling Reporter; 3.8 Conclusion; References; 4 Electrochemical Sandwich Assays for Protein Detection; Abstract; 4.1 Introduction; 4.2 Immunoassays; 4.2.1 Enzyme Amplification; 4.2.2 Metal Nanoparticles Amplification; 4.2.3 Carbon-Based Nanomaterial Amplification; 4.2.4 Magnetic Beads and Magnetic Nanoparticles.

4.2.5 Other Amplification Strategies4.3 Aptamer-Based Sandwich Assays; 4.3.1 Configuration 1: Dual-Aptamer Binding Configuration; 4.3.2 Configuration 2 and 3: Aptamerâ#x80;#x93;Antibody Configuration; 4.4 Conclusion; References; 5 Sandwich Assays Based on SPR, SERS, GMR, QCM, Microcantilever, SAW, and RRS Techniques for Protein Detection; Abstract; 5.1 Introduction; 5.2 Sandwich Assays Based on SPR; 5.3 Sandwich Assays Based on SERS; 5.4 Sandwich Assays Based on GMR; 5.5 Sandwich Assays Based on QCM; 5.6 Sandwich Assays Based on Microcantilever; 5.7 Sandwich Assays Based on SAW and RRS.

5.8 ConclusionReferences; 6 Colorimetric Sandwich Assays for Nucleic Acid Detection; Abstract; 6.1 Introduction; 6.2 Colorimetric Sandwich Assays Based on Gold Nanoparticles; 6.3 Colorimetric Sandwich Assays Based on Traditional Enzymes; 6.4 Colorimetric Sandwich Assays Based on DNAzyme; 6.5 Conclusion; References; 7 Fluorescence Sandwich Assays for Nucleic Acid Detection; Abstract; 7.1 Introduction; 7.2 Organic Dyes as Fluorophores for Sandwich Assays; 7.2.1 Sandwich Assays Based on Fluorescence Resonance Energy Transfer (FRET).

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