@article{826902,
      recid = {826902},
      author = {Sasakura, Yasunori,},
      title = {Transgenic ascidians /},
      pages = {1 online resource.},
      abstract = {This book comprehensively describes the transgenesis  techniques and applied experimental methods in ascidians  including enthusiastically developed original devices in  addition to concrete examples of developmental biology  studies. Ascidians have been one of the most important  model animals in developmental biology for studying  molecular and cellular processes underlying formation of  the chordate body plan. Transgenic techniques such as  microinjection, electropolation, cis-element analysis and  application, and TALENs and CRISPR/Cas9 have been developed  in ascidians for more than 20 years, and now many applied  methods, some of which are unique in ascidians, have been  accumulated. Those extensive technological innovations,  such as cell isolation, cell labeling, germ-line  transformation, marker transgenic lines, and the  experimental systems for studying notochord formation and  nervous system, are exceptional particularly in marine  invertebrates. This book is useful for ascidian researchers  to quickly access the techniques in which they are  interested as well as to compare each technology to become  familiar with specialized tips, and for biologists of other  organisms to learn the unique techniques and ingenious  attempts specific to ascidians. Providing detailed and  easily understandable descriptions of techniques, the book  will inspire ascidian specialists to improve their  techniques, encourage anyone wanting to begin studying  ascidians, and enable readers to immediately apply the  techniques to the organisms they research.},
      url = {http://library.usi.edu/record/826902},
      doi = {https://doi.org/10.1007/978-981-10-7545-2},
}