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000826902 019__ $$a1029239553$$a1029343264$$a1029444446$$a1034628543
000826902 020__ $$a9789811075452$$q(electronic book)
000826902 020__ $$a981107545X$$q(electronic book)
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000826902 0247_ $$a10.1007/978-981-10-7545-2$$2doi
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000826902 049__ $$aISEA
000826902 050_4 $$aQH442.6
000826902 08204 $$a571.8$$223
000826902 24500 $$aTransgenic ascidians /$$cYasunori Sasakura, editor.
000826902 264_1 $$aSingapore, Singapore :$$bSpringer,$$c[2018]
000826902 300__ $$a1 online resource.
000826902 336__ $$atext$$btxt$$2rdacontent
000826902 337__ $$acomputer$$bc$$2rdamedia
000826902 338__ $$aonline resource$$bcr$$2rdacarrier
000826902 347__ $$atext file$$bPDF$$2rda
000826902 4901_ $$aAdvances in experimental medicine and biology,$$x0065-2598 ;$$vvolume 1029
000826902 504__ $$aIncludes bibliographical references and index.
000826902 5050_ $$aIntroduction / Yasunori Sasakura -- Microinjection of exogenous nucleic acids into eggs: Ciona species / Kenji Kobayashi, Yutaka Satou -- Practical guide for ascidian microinjection: Phallusia mammillata / Hitoyoshi Yasuo, Alex McDougall -- Microinjection of exogenous DNA into eggs of Halocynthia roretzi / Gaku Kumano -- Electroporation in ascidians: history, theory and protocols / Robert W. Zeller -- The use of cis-regulatory DNAs as molecular tools / Kotaro Shimai, Takehiro G. Kusakabe -- Reporter analyses reveal redundant enhancers that confer robustness on cis-regulatory mechanisms / Shigeki Fujiwara, Cristian Cañestro -- investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord / Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio -- Purification of fluorescent labeled cells from dissociated Ciona embryos / Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen -- Germline transgenesis in Ciona / Yasunori Sasakura -- The enhancer trap in Ciona / Yasunori Sasakura -- TALEN-based knockout system / Keita Yoshida, Nicholas Treen -- CRISPR knockouts in Ciona embryos / Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi -- Transgenic Techniques for Investigating Cell Biology During Development / Christina D. Cota -- Cellular processes of notochord formation / William C. Smith -- Morphology and physiology of the ascidian nervous systems and the effectors / Atsuo Nishino.
000826902 506__ $$aAccess limited to authorized users.
000826902 520__ $$aThis book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.
000826902 588__ $$aOnline resource; title from PDF title page (SpringerLink, viewed March 21, 2018).
000826902 650_0 $$aTransgenic organisms.
000826902 650_0 $$aSea squirts.
000826902 650_0 $$aCytology.
000826902 650_0 $$aDevelopmental biology.
000826902 650_0 $$aEvolution (Biology)
000826902 650_0 $$aBiology$$xTechnique.
000826902 7001_ $$aSasakura, Yasunori,$$eeditor.
000826902 77608 $$iPrint version: $$z9811075441$$z9789811075445$$w(OCoLC)1011236059
000826902 830_0 $$aAdvances in experimental medicine and biology ;$$vv. 1029.
000826902 852__ $$bebk
000826902 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-981-10-7545-2$$zOnline Access$$91397441.1
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000826902 980__ $$aEBOOK
000826902 980__ $$aBIB
000826902 982__ $$aEbook
000826902 983__ $$aOnline
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