000839565 000__ 03734cam\a2200457Ii\4500 000839565 001__ 839565 000839565 005__ 20230306144736.0 000839565 006__ m\\\\\o\\d\\\\\\\\ 000839565 007__ cr\un\nnnunnun 000839565 008__ 180531s2018\\\\sz\a\\\\ob\\\\001\0\eng\d 000839565 019__ $$a1038392059$$a1040613074 000839565 020__ $$a9783319779829$$q(electronic book) 000839565 020__ $$a3319779826$$q(electronic book) 000839565 020__ $$z9783319777627 000839565 0247_ $$a10.1007/978-3-319-77982-9$$2doi 000839565 035__ $$aSP(OCoLC)on1038068092 000839565 035__ $$aSP(OCoLC)1038068092$$z(OCoLC)1038392059$$z(OCoLC)1040613074 000839565 040__ $$aN$T$$beng$$erda$$epn$$cN$T$$dN$T$$dGW5XE$$dEBLCP$$dAZU$$dUAB$$dYDX$$dOCLCF$$dOCLCQ 000839565 049__ $$aISEA 000839565 050_4 $$aQH442.2 000839565 08204 $$a572.8$$223 000839565 1001_ $$aWong, Dominic W. S,$$eauthor. 000839565 24514 $$aThe ABCs of gene cloning /$$cDominic W.S. Wong. 000839565 250__ $$aThird edition. 000839565 264_1 $$aCham, Switzerland :$$bSpringer,$$c[2018] 000839565 300__ $$a1 online resource :$$billustrations 000839565 336__ $$atext$$btxt$$2rdacontent 000839565 337__ $$acomputer$$bc$$2rdamedia 000839565 338__ $$aonline resource$$bcr$$2rdacarrier 000839565 347__ $$atext file$$bPDF$$2rda 000839565 504__ $$aIncludes bibliographical references and index. 000839565 5050_ $$aFundamentals of Genetic Processes -- Introductory Concepts -- Structures of Nucleic Acids -- Structures of Proteins -- The Genetic Process -- Organization of Genes -- Reading The Nucleotide Sequence of a Gene -- Techniques and Strategies of Gene Cloning -- Enzymes Used in Cloning -- Techniques Used in Cloning -- Cloning Vectors for Introducing Genes into Host Cells -- Transformation -- Isolating Genes for Cloning -- Impact of Gene Cloning -- Improving Tomato Quality by Antisense Rna -- Transgenic Crops Engineered with Insecticidal Activity -- Transgenic Crops Conferred with Herbicide Resistance -- Growth Enhancement in Transgenic Fish -- Impact of Gene Cloning -- Microbial Production of Recombinant Human Insulin -- Finding Disease-Causing Genes -- Human Gene Therapy -- Gene Targeting -- DNA Typing -- Transpharmers -- Bioreactors for Pharmaceutical Products -- Animal Cloning -- Human Genome Sequencing -- Manipulating Gene Vector Constructs. 000839565 506__ $$aAccess limited to authorized users. 000839565 520__ $$aClear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas. The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning. 000839565 588__ $$aOnline resource; title from PDF title page (viewed June 01, 2018). 000839565 650_0 $$aMolecular cloning. 000839565 77608 $$iPrint version: $$z9783319777627 000839565 852__ $$bebk 000839565 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-319-77982-9$$zOnline Access$$91397441.1 000839565 909CO $$ooai:library.usi.edu:839565$$pGLOBAL_SET 000839565 980__ $$aEBOOK 000839565 980__ $$aBIB 000839565 982__ $$aEbook 000839565 983__ $$aOnline 000839565 994__ $$a92$$bISE