Microarray analysis : biochips and eradication of all diseases / Kal Renganathan Sharma.
Sharma, Kal Renganathan., author.
2015
QP624.5.D726 S526 2015
Available Online

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Title
Microarray analysis : biochips and eradication of all diseases / Kal Renganathan Sharma.
Author
Sharma, Kal Renganathan., author.
ISBN
9781606506707 (electronic book)
9781606506677 print
Published
New York, [New York] (222 East 46th Street, New York, NY 10017) : Momentum Press, 2015.
Language
English
Description
1 online resource (xxviii, 295 pages) : illustrations.
Call Number
QP624.5.D726 S526 2015
Dewey Decimal Classification
572.8636
Summary
Microarray Analysis is a natural outgrowth of the author's teaching and research into the field of microarray processing, a fast growing and developing field. The book highlights the development of various microarray analysis tools and the applications of the technology towards disease prevention and eradication. The goal is to illustrate the many sequencing methods in place, and to show the various applications of these methods. This book will show how the biochemical reactions involved in the different steps of the cure process can be studied using microarray methods. Examples will be provided throughout the text of various disease states, including the spread of cystic fibrosis in children consanguineous marriages in Saudi Arabia, genetic disorders, and autoimmune disorders. The protocols from sample preparation to confocal scanning microscopy and detectors using photo multiplier tubes, biochip manufacturing methods, advances in sequencing methods, microfluids, electrophoretic methods are discussed in detail. The Polymerase chain reaction, a technique used to amplify and simultaneously quantify a targeted DNA molecule is explored. The criteria for choosing the best surface to use for microarray analysis is elloborated. Additionally, Microarray Analysis: Biochips and Eradication of Disease will feature the statistical methods needed for analysis of DNA data from microarray analysis, including shotgun and next generation sequencing.
Bibliography, etc. Note
Includes bibliographical references (pages 271-282) and index.
Access Note
Access limited to authorized users.
Source of Description
Title from PDF title page (viewed on July 9, 2015).
Series
Biomedical engineering collection.
Available in Other Form
Print version: 9781606506677
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Online Access
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1. Genominomics and prospects
Chapter objectives
Human genome project completed
Biochips
Next-generation sequencing
Applications of biochips
Consanguinous marriages
Ashkenazi Jews
Cystic fibrosis
Tuberculosis
Cancer
Sickle-cell anemia
Small pox
Paleo genomes
Colony collapse disorder
Schizophrenia
Prenatal testing
DNA for forensic investigation
Genomic surveillance
Bio-corrosion
Human immunodeficiency syndrome
Microarray analysis
Pioneers in double-helix structure of DNA
Genominomics, economics of sequencing, $1,000
Genome is within reach
Genetically modified crops
Glossary
Summary


2. Microarray analysis
Chapter objectives
Molecular basis for disease
Microarray technology development
Specifications
Nanoprint microarrayer
Ten tips for five-step process
Step 1 formulation of a biologic question
Step 2 sample preparation
Step 3 biochemical reaction
Step 4 microarray detection
Step 5 microarray data analysis and modeling
Microarray fluorescence detection
Confocal scanning microscope
Quality of substrate surface
Phosphoramadite synthesis
Summary


3. Sequencing technology advances
Chapter objectives
Genomes completed
Next-generation sequencers
Gene mapping
Electrophoretic methods
Microfluidic separations
Polymer liquids
Transport parameters
Transient concentration profile predicted using the a capite ad calcem concentration non-Fick diffusion equation in a semi-infinite medium
Convection and diffusion
Summary


4. Applications
Chapter objectives
Cancer
Kinetic model for progression of cancer
Proteomics and Michaelis and Menten kinetics
Immune action mechanism
DNA hybridization kinetics-diffusion effects
Gene modifications
DNA-melting temperature
Genetic disorders and microarray analysis
Tissue microarrays and cell microarrays
Bio-based polymers
Gene silencing
Metabolomics
Recombinant DNA technology and genetically
Modified crops
Biodiesel
Consecutive-competitive reactions
Centrifugal separation of fame and glycerol: torque requirements
Shear flow theory
Results
Gene therapy
Gene activity in songbirds similar to humans
Glossary
Summary


5. Next-generation sequencing
Chapter objectives
Blotting techniques
Sanger sequencing
Sequencing by synthesis
Sequencing by ligation
Pyrosequencing
Single-molecule sequencing
DNA sequencing through nanopore
Glossary
Summary


6. Biochip manufacturing
Chapter objectives
Three approaches
Ex situ manufacturing
Commercial instruments
Time to print
Summary


7. Statistical characterization and normalization
Chapter objectives
Housekeeping genes and normalization
Clustering
Supervised
Variation filter
Unsupervised
Pearson's correlation coefficient
Principal component analysis
Cluster determination
Nearest neighbor clustering
Unsupervised classification
Silhouette method
Dunn's validation index
Davies-Bouldin index
C-index
Self-organizing maps
K-means clustering
Agglomerative clustering
Dendrograms
Two-dimensional dendrograms
Division or partition clustering
Bayesian clustering
Boolean networks
Bayesian networks
Relevance networks
Glossary
Summary


References
About the author
Index.

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