000841853 000__ 08070cam\a2201009\i\4500 000841853 001__ 841853 000841853 005__ 20210515152009.0 000841853 006__ m\\\\\o\\d\\\\\\\\ 000841853 007__ cr\cn\nnnunnun 000841853 008__ 150709s2015\\\\nyua\\\foab\\\001\0\eng\d 000841853 020__ $$a9781606506707$$q(electronic book) 000841853 020__ $$z9781606506677$$qprint 000841853 035__ $$a(MiAaPQ)EBC3563988 000841853 035__ $$a(Au-PeEL)EBL3563988 000841853 035__ $$a(CaPaEBR)ebr11071696 000841853 035__ $$a(CaONFJC)MIL801563 000841853 035__ $$a(OCoLC)913604543 000841853 040__ $$aFINmELB$$bspa$$erda$$cFINmELB 000841853 050_4 $$aQP624.5.D726$$bS526 2015 000841853 0820_ $$a572.8636$$223 000841853 1001_ $$aSharma, Kal Renganathan.,$$eauthor. 000841853 24510 $$aMicroarray analysis :$$bbiochips and eradication of all diseases /$$cKal Renganathan Sharma. 000841853 264_1 $$aNew York, [New York] (222 East 46th Street, New York, NY 10017) :$$bMomentum Press,$$c2015. 000841853 300__ $$a1 online resource (xxviii, 295 pages) :$$billustrations. 000841853 336__ $$atext$$2rdacontent 000841853 337__ $$acomputer$$2rdamedia 000841853 338__ $$aonline resource$$2rdacarrier 000841853 4901_ $$aBiomedical engineering collection 000841853 504__ $$aIncludes bibliographical references (pages 271-282) and index. 000841853 5050_ $$a1. Genominomics and prospects -- Chapter objectives -- Human genome project completed -- Biochips -- Next-generation sequencing -- Applications of biochips -- Consanguinous marriages -- Ashkenazi Jews -- Cystic fibrosis -- Tuberculosis -- Cancer -- Sickle-cell anemia -- Small pox -- Paleo genomes -- Colony collapse disorder -- Schizophrenia -- Prenatal testing -- DNA for forensic investigation -- Genomic surveillance -- Bio-corrosion -- Human immunodeficiency syndrome -- Microarray analysis -- Pioneers in double-helix structure of DNA -- Genominomics, economics of sequencing, $1,000 -- Genome is within reach -- Genetically modified crops -- Glossary -- Summary -- 000841853 5058_ $$a2. Microarray analysis -- Chapter objectives -- Molecular basis for disease -- Microarray technology development -- Specifications -- Nanoprint microarrayer -- Ten tips for five-step process -- Step 1 formulation of a biologic question -- Step 2 sample preparation -- Step 3 biochemical reaction -- Step 4 microarray detection -- Step 5 microarray data analysis and modeling -- Microarray fluorescence detection -- Confocal scanning microscope -- Quality of substrate surface -- Phosphoramadite synthesis -- Summary -- 000841853 5058_ $$a3. Sequencing technology advances -- Chapter objectives -- Genomes completed -- Next-generation sequencers -- Gene mapping -- Electrophoretic methods -- Microfluidic separations -- Polymer liquids -- Transport parameters -- Transient concentration profile predicted using the a capite ad calcem concentration non-Fick diffusion equation in a semi-infinite medium -- Convection and diffusion -- Summary -- 000841853 5058_ $$a4. Applications -- Chapter objectives -- Cancer -- Kinetic model for progression of cancer -- Proteomics and Michaelis and Menten kinetics -- Immune action mechanism -- DNA hybridization kinetics-diffusion effects -- Gene modifications -- DNA-melting temperature -- Genetic disorders and microarray analysis -- Tissue microarrays and cell microarrays -- Bio-based polymers -- Gene silencing -- Metabolomics -- Recombinant DNA technology and genetically -- Modified crops -- Biodiesel -- Consecutive-competitive reactions -- Centrifugal separation of fame and glycerol: torque requirements -- Shear flow theory -- Results -- Gene therapy -- Gene activity in songbirds similar to humans -- Glossary -- Summary -- 000841853 5058_ $$a5. Next-generation sequencing -- Chapter objectives -- Blotting techniques -- Sanger sequencing -- Sequencing by synthesis -- Sequencing by ligation -- Pyrosequencing -- Single-molecule sequencing -- DNA sequencing through nanopore -- Glossary -- Summary -- 000841853 5058_ $$a6. Biochip manufacturing -- Chapter objectives -- Three approaches -- Ex situ manufacturing -- Commercial instruments -- Time to print -- Summary -- 000841853 5058_ $$a7. Statistical characterization and normalization -- Chapter objectives -- Housekeeping genes and normalization -- Clustering -- Supervised -- Variation filter -- Unsupervised -- Pearson's correlation coefficient -- Principal component analysis -- Cluster determination -- Nearest neighbor clustering -- Unsupervised classification -- Silhouette method -- Dunn's validation index -- Davies-Bouldin index -- C-index -- Self-organizing maps -- K-means clustering -- Agglomerative clustering -- Dendrograms -- Two-dimensional dendrograms -- Division or partition clustering -- Bayesian clustering -- Boolean networks -- Bayesian networks -- Relevance networks -- Glossary -- Summary -- 000841853 5058_ $$aReferences -- About the author -- Index. 000841853 506__ $$aAccess limited to authorized users. 000841853 5203_ $$aMicroarray Analysis is a natural outgrowth of the author's teaching and research into the field of microarray processing, a fast growing and developing field. The book highlights the development of various microarray analysis tools and the applications of the technology towards disease prevention and eradication. The goal is to illustrate the many sequencing methods in place, and to show the various applications of these methods. This book will show how the biochemical reactions involved in the different steps of the cure process can be studied using microarray methods. Examples will be provided throughout the text of various disease states, including the spread of cystic fibrosis in children consanguineous marriages in Saudi Arabia, genetic disorders, and autoimmune disorders. The protocols from sample preparation to confocal scanning microscopy and detectors using photo multiplier tubes, biochip manufacturing methods, advances in sequencing methods, microfluids, electrophoretic methods are discussed in detail. The Polymerase chain reaction, a technique used to amplify and simultaneously quantify a targeted DNA molecule is explored. The criteria for choosing the best surface to use for microarray analysis is elloborated. Additionally, Microarray Analysis: Biochips and Eradication of Disease will feature the statistical methods needed for analysis of DNA data from microarray analysis, including shotgun and next generation sequencing. 000841853 588__ $$aTitle from PDF title page (viewed on July 9, 2015). 000841853 650_0 $$aDNA microarrays. 000841853 653__ $$aGenominomics 000841853 653__ $$aNext Generation Sequencing 000841853 653__ $$aMicroarray Analysis 000841853 653__ $$aCancer Treatment 000841853 653__ $$aCancer Progression 000841853 653__ $$aGene Silencing 000841853 653__ $$aSingle Nucleotide Polymorphism 000841853 653__ $$aGenetic Disorders 000841853 653__ $$aAutoimmune Disorders 000841853 653__ $$aGenetically Modified Crops 000841853 653__ $$aSmall Pox 000841853 653__ $$aMolecular Basis for Disease 000841853 653__ $$aBiochemical Reactions 000841853 653__ $$aMultiple Reactions 000841853 653__ $$aConfocal Scanners 000841853 653__ $$aPhosphoramidites 000841853 653__ $$aElectrophoretic Methods 000841853 653__ $$aMicrofluidic Separations 000841853 653__ $$aGene Mapping 000841853 653__ $$aPolymer Liquids 000841853 653__ $$aRelaxation Phenomena 000841853 653__ $$aGene Modifications 000841853 653__ $$aSplicing 000841853 653__ $$aGenomics 000841853 653__ $$aProteomics 000841853 653__ $$aMetabolomics 000841853 653__ $$aJ. Curcas 000841853 653__ $$aBiodiesel 000841853 653__ $$aBlotting 000841853 653__ $$aSanger Sequencing 000841853 653__ $$aSequencing by Synthesis 000841853 653__ $$aSequencing by Ligation 000841853 653__ $$aPyrosequencing 000841853 653__ $$aSingle Molecule Sequencing 000841853 653__ $$aNanopore 000841853 653__ $$aBiochip Manufacturing 000841853 653__ $$aHouse Keeping Genes 000841853 653__ $$aClustering 000841853 653__ $$ak means Clustering 000841853 653__ $$aBaysean Networks 000841853 655_4 $$aLibros electronicos. 000841853 77608 $$iPrint version:$$z9781606506677 000841853 830_0 $$aBiomedical engineering collection. 000841853 852__ $$bebk 000841853 85640 $$3ProQuest Ebook Central Academic Complete$$uhttps://univsouthin.idm.oclc.org/login?url=https://ebookcentral.proquest.com/lib/usiricelib-ebooks/detail.action?docID=3563988$$zOnline Access 000841853 909CO $$ooai:library.usi.edu:841853$$pGLOBAL_SET 000841853 980__ $$aEBOOK 000841853 980__ $$aBIB 000841853 982__ $$aEbook 000841853 983__ $$aOnline