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Intro; Preface; For Gram +ve Bacterium; PCR Amplification of 16S Ribosomal RNA Gene; PCR Product Purification and Sequencing; 16S rRNA Gene Sequence Homology and Phylogeny Analysis; Sequence Submission and Culture Deposition; Molecular Characterization of Plant Growth Promoting Bacterium and Phylogeny Analysis; Evolutionary Relationships of Bacillus sp. SJ-5; Molecular Characterization of Plant Growth Promoting Properties of Bacteria; For Grame -ve Bacterium; Gene Amplification; Gene Sequencing and Analysis; Phylogeny Based on 16 rRNA Gene Analyses; Phylogeny Analysis of PGP Properties Genes
About the Editors; Chapter 1: Genes to Metabolites and Metabolites to Genes Approaches to Predict Biosynthetic Pathways in Microbes for Natural P...; 1.1 Introduction; 1.2 Why Microbial Secondary Metabolites Are Needed?; 1.3 Genome-Based Mining Strategies; 1.4 Retrograde Pathway Evolution; 1.5 Pathway Evolution Through Retrobiosynthesis and Retrosynthetic Designs; 1.6 Genome-Based Mining Tools for Genes to Metabolites (Forward) Approach; 1.7 Databases Dedicated to Secondary Metabolite Biosynthetic Gene Clusters
1.8 Genome-Based Mining Tools for Metabolites to Genes (Retrosynthetic) Approach1.9 Concluding Remarks; References; Chapter 2: Role of Functional Bacterial Phylum Proteobacteria in Glycine max Growth Promotion Under Abiotic Stress: A Glimpse ...; 2.1 Introduction; 2.2 Soybean Numero Uno Crop; 2.2.1 Selection and Characterization of PGPR Strains; 2.2.2 PGPR Characteristics: Case Studies; 2.2.2.1 Qualitative and Quantitative Estimation of Phosphate Solubilization Under Salt; 2.2.2.2 ACC Deaminase Activity in Different Salt Concentrations
2.2.2.3 Proline Determination in Different Salt Concentrations2.2.2.4 Exopolysaccharide Production; 2.2.2.5 Molecular Characterization of Genes in Bacteria; 2.2.3 Sterilization and Viability and Seed Sensitivity; 2.2.4 Standardization for Bacterial Inoculums; 2.2.4.1 Bacterial Growth Under Stress Conditions; 2.2.5 PGP Traits; 2.2.5.1 IAA Production on Different Salt Concentration; 2.2.5.2 Phosphate Solubilization on Different Salt Concentration; 2.2.5.3 Siderophore Production on Different Salt Concentration; 2.2.5.4 ACC Deaminase Activity on Different Salt Concentration
2.2.5.5 EPS Production on Different Salt Stress2.2.5.6 Phylogeny Analysis of PGP Gene Properties; References; Chapter 3: Methanogenic Archaea in Paddy Agricultural Fields; 3.1 Introduction; 3.2 Materials and Methods; 3.2.1 Experimental Site and Soil Sampling; 3.2.2 Soil Physicochemical Analyses; 3.2.3 Methane Production Potential of Soil; 3.2.4 Kinetics of Methane Production; 3.2.5 PCR-Cloning-RFLP Analysis, Sequencing, and Assignment of Clones into OTUs; 3.2.6 Nucleotide Sequence Accession Numbers; 3.2.7 Diversity Indices; 3.3 Results and Discussion
About the Editors; Chapter 1: Genes to Metabolites and Metabolites to Genes Approaches to Predict Biosynthetic Pathways in Microbes for Natural P...; 1.1 Introduction; 1.2 Why Microbial Secondary Metabolites Are Needed?; 1.3 Genome-Based Mining Strategies; 1.4 Retrograde Pathway Evolution; 1.5 Pathway Evolution Through Retrobiosynthesis and Retrosynthetic Designs; 1.6 Genome-Based Mining Tools for Genes to Metabolites (Forward) Approach; 1.7 Databases Dedicated to Secondary Metabolite Biosynthetic Gene Clusters
1.8 Genome-Based Mining Tools for Metabolites to Genes (Retrosynthetic) Approach1.9 Concluding Remarks; References; Chapter 2: Role of Functional Bacterial Phylum Proteobacteria in Glycine max Growth Promotion Under Abiotic Stress: A Glimpse ...; 2.1 Introduction; 2.2 Soybean Numero Uno Crop; 2.2.1 Selection and Characterization of PGPR Strains; 2.2.2 PGPR Characteristics: Case Studies; 2.2.2.1 Qualitative and Quantitative Estimation of Phosphate Solubilization Under Salt; 2.2.2.2 ACC Deaminase Activity in Different Salt Concentrations
2.2.2.3 Proline Determination in Different Salt Concentrations2.2.2.4 Exopolysaccharide Production; 2.2.2.5 Molecular Characterization of Genes in Bacteria; 2.2.3 Sterilization and Viability and Seed Sensitivity; 2.2.4 Standardization for Bacterial Inoculums; 2.2.4.1 Bacterial Growth Under Stress Conditions; 2.2.5 PGP Traits; 2.2.5.1 IAA Production on Different Salt Concentration; 2.2.5.2 Phosphate Solubilization on Different Salt Concentration; 2.2.5.3 Siderophore Production on Different Salt Concentration; 2.2.5.4 ACC Deaminase Activity on Different Salt Concentration
2.2.5.5 EPS Production on Different Salt Stress2.2.5.6 Phylogeny Analysis of PGP Gene Properties; References; Chapter 3: Methanogenic Archaea in Paddy Agricultural Fields; 3.1 Introduction; 3.2 Materials and Methods; 3.2.1 Experimental Site and Soil Sampling; 3.2.2 Soil Physicochemical Analyses; 3.2.3 Methane Production Potential of Soil; 3.2.4 Kinetics of Methane Production; 3.2.5 PCR-Cloning-RFLP Analysis, Sequencing, and Assignment of Clones into OTUs; 3.2.6 Nucleotide Sequence Accession Numbers; 3.2.7 Diversity Indices; 3.3 Results and Discussion