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000844696 020__ $$a9781615047857$$q(electronic book)
000844696 020__ $$a1615047859$$q(electronic book)
000844696 020__ $$z9781615047864
000844696 020__ $$z9781615047840
000844696 0247_ $$a10.4199/C00164ED1V01Y201805QCB004$$2doi
000844696 035__ $$a(NhCcYBP)EBC5476840
000844696 040__ $$aNhCcYBP$$cNhCcYBP
000844696 050_4 $$aQH634$$b.B476 2018
000844696 08204 $$a571.655$$223
000844696 1001_ $$aBerro, Julien,$$eauthor.
000844696 24510 $$aQuantitative biology of endocytosis /$$cJulien Berro, Michael M. Lacy.
000844696 264_1 $$a[San Rafael, California] :$$bMorgan & Claypool,$$c2018.
000844696 300__ $$a1 online resource (xi, 73 pages) :$$billustrations.
000844696 336__ $$atext$$2rdacontent
000844696 337__ $$aelectronic$$2isbdmedia
000844696 338__ $$aonline resource$$2rdacarrier
000844696 4901_ $$aColloquium series on quantitive cell biology,$$x2375-7752 ;$$v# 4
000844696 500__ $$aPart of: Colloquium digital library of life sciences.
000844696 504__ $$aIncludes bibliographical references (pages 61-72).
000844696 5050_ $$a1. Introduction to clathrin-mediated endocytosis -- 1.1 Proteins involved in CME -- Membrane coat proteins -- Endocytic actin meshwork -- Membrane scission and late regulation -- 1.2 Membrane in CME -- 1.3 Forces in CME -- 1.4 Comparison of CME in yeast and mammals -- 1.5 CME in human health and disease -- 1.6 Understanding CME requires quantitative approaches -- 1.7 Conclusions --
000844696 5058_ $$a2. Collecting quantitative data -- 2.1 Using fluorescence microscopy to obtain quantitative data about endocytosis -- Overview of fluorescence microscopes that can be used for quantitative analysis of endocytosis -- Comparison of different fluorescence microscopy systems -- General considerations about fluorescent tags -- 2.2 Practical considerations for counting molecules using a fluorescence microscope -- Tag a protein of interest at its genomic locus -- Limit photobleaching -- Limit uneven illumination of the field of view -- Ensure the microscopy system is used in conditions where the signal is linear -- Calibrate the microscope using flexible settings to avoid calibrating too often -- Best practices to limit sources of variability -- 2.3 Electron microscopy and correlative light and electron microscopy (CLEM) -- 2.4 Super-resolution microscopy --
000844696 5058_ $$a3. From raw images to quantitative measurements: extracting, correcting, and aligning the fluorescence microscopy data -- 3.1 Tracking endocytic events -- Challenges in tracking endocytic events -- Tracking and track curation -- Best practice in tracking and curating tracks -- 3.2 Processing the data to count molecules -- Pre-processing of the movies -- Correcting for cytoplasmic background -- 3.3 Aligning the tracking data -- Simple but biased alignment methods -- Advanced and more accurate alignment methods -- 3.4 Counting the number of endocytic events --
000844696 5058_ $$a4. Using quantitative microscopy data to infer the molecular mechanisms of endocytosis -- 4.1 How are endocytic proteins organized? -- The protein copy numbers and their stoichiometries constrain possible molecular organizations and mechanisms -- High-resolution track alignment can infer the organization of endocytic proteins -- The organization of the actin meshwork around the endocytic vesicle can be inferred from the vesicle's movements -- 4.2 What are the molecular mechanisms of actin dynamics during endocytosis? -- Inferring mechanisms by comparing numbers and stoichiometries -- Mathematical modeling constrained by quantitative data allows one to test different mechanisms and make testable predictions -- 4.3 What are the mechanical properties of the membrane and the forces involved in membrane deformations? -- Modeling of membrane shapes can elucidate mechanical properties and forces involved during CME -- Simulations to test possible force distributions to elongate the CCP and propose new mechanisms --
000844696 5058_ $$a5. Perspectives and future of quantitative biology of endocytosis -- 5.1 Open questions in CME -- 5.2 Quantities that are not (yet?) measurable -- 5.3 Advances in microscopy techniques -- 5.4 Advances in computational tools -- 5.5 Applications to other systems --
000844696 5058_ $$aReferences -- Author biographies.
000844696 506__ $$aAccess limited to authorized users
000844696 5100_ $$aGoogle scholar
000844696 5100_ $$aGoogle book search
000844696 533__ $$aElectronic reproduction.$$bAnn Arbor, MI$$nAvailable via World Wide Web.
000844696 588__ $$aTitle from PDF title page (viewed on August 3, 2018).
000844696 650_0 $$aEndocytosis.
000844696 7001_ $$aLacy, Michael M.,$$eauthor.
000844696 7102_ $$aProQuest (Firm)
000844696 77608 $$iPrint version:$$z9781615047840$$z9781615047864
000844696 830_0 $$aColloquium digital library of life sciences.
000844696 830_0 $$aColloquium series on quantitive cell biology ;$$v# 4.
000844696 852__ $$bebk
000844696 85640 $$3GOBI DDA$$uhttps://univsouthin.idm.oclc.org/login?url=https://ebookcentral.proquest.com/lib/usiricelib-ebooks/detail.action?docID=5476840$$zOnline Access
000844696 909CO $$ooai:library.usi.edu:844696$$pGLOBAL_SET
000844696 980__ $$aEBOOK
000844696 980__ $$aBIB
000844696 982__ $$aEbook
000844696 983__ $$aOnline