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2.1 Structure and Quantum Yield Relationship Study of Seoul-Fluor2.1.1 Introduction; 2.1.2 Result and Discussion; 2.1.3 Conclusion; 2.2 Optimization of SF-Based Lipid Droplet Bioprobes on the Basis of Molecular Lipophilicity; 2.2.1 Introduction; 2.2.2 Result and Discussion; 2.2.3 Conclusion; 2.3 An Overview of Seoul-Fluor System; 2.4 Experimental Section; 2.4.1 General Experimental Information; 2.4.2 Experimental Procedure; 2.4.3 Compound Characterization Data; References; 3 Tetrazine-Containing Colorful Bioorthogonal Probes Based on the Indolizine Core Skeleton; 3.1 Introduction
3.2 Result and Discussion3.2.1 Initial Design and Synthesis; 3.2.2 Change in Absorption Property of SFTz01-05 Upon TCO Cycloaddition Reaction; 3.2.3 Change in Fluorescence Property of SFTz01-05 Upon Cycloaddition Reaction with TCO; 3.2.4 Reaction Kinetics; 3.2.5 Rational Expansion of Multicolor SFTzs Based on a Monochromophoric Strategy; 3.2.6 TD-DFT Calculation; 3.2.7 Direct Comparison of Mono- Versus Bichromophoric SFTzs with Long Emission Wavelength; 3.2.8 Bioapplication; 3.3 Conclusion; 3.4 Experimental Section; 3.4.1 General Experimental Information
3.4.2 Fluorescence Kinetic Measurements3.4.3 Experimental Procedure for Live Cell Fluorescence Image; 3.4.4 Synthetic Procedure and Characterization of New Compounds; References; 4 Rational Development of Furoindolizine Core Skeleton Guided by Oscillator Strength; 4.1 Introduction; 4.2 Result and Discussion; 4.2.1 Designing an Unexplored Fluorophore with High Molar Absorptivity; 4.2.2 Library Construction Based on a Novel Furo[3,2-e]Indolizine Scaffold; 4.2.3 Photophysical Property Analysis; 4.2.4 Rational Design of Novel Furoindolizine-Based Fluorescent Compounds and Bioapplication
4.3 Conclusion4.4 Experimental Section; 4.4.1 General Information; 4.4.2 Experimental Procedure for Live Cell Fluorescence Image; 4.4.3 Computational Results of Furoindolizine Analogues; 4.4.4 Prediction of Photophysical Properties for 17, 18 and 19; 4.4.5 Synthetic Procedure and Compound Characterization; References; Appendix; Chapter 2; Chapter 3; Chapter 4
3.2 Result and Discussion3.2.1 Initial Design and Synthesis; 3.2.2 Change in Absorption Property of SFTz01-05 Upon TCO Cycloaddition Reaction; 3.2.3 Change in Fluorescence Property of SFTz01-05 Upon Cycloaddition Reaction with TCO; 3.2.4 Reaction Kinetics; 3.2.5 Rational Expansion of Multicolor SFTzs Based on a Monochromophoric Strategy; 3.2.6 TD-DFT Calculation; 3.2.7 Direct Comparison of Mono- Versus Bichromophoric SFTzs with Long Emission Wavelength; 3.2.8 Bioapplication; 3.3 Conclusion; 3.4 Experimental Section; 3.4.1 General Experimental Information
3.4.2 Fluorescence Kinetic Measurements3.4.3 Experimental Procedure for Live Cell Fluorescence Image; 3.4.4 Synthetic Procedure and Characterization of New Compounds; References; 4 Rational Development of Furoindolizine Core Skeleton Guided by Oscillator Strength; 4.1 Introduction; 4.2 Result and Discussion; 4.2.1 Designing an Unexplored Fluorophore with High Molar Absorptivity; 4.2.2 Library Construction Based on a Novel Furo[3,2-e]Indolizine Scaffold; 4.2.3 Photophysical Property Analysis; 4.2.4 Rational Design of Novel Furoindolizine-Based Fluorescent Compounds and Bioapplication
4.3 Conclusion4.4 Experimental Section; 4.4.1 General Information; 4.4.2 Experimental Procedure for Live Cell Fluorescence Image; 4.4.3 Computational Results of Furoindolizine Analogues; 4.4.4 Prediction of Photophysical Properties for 17, 18 and 19; 4.4.5 Synthetic Procedure and Compound Characterization; References; Appendix; Chapter 2; Chapter 3; Chapter 4