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000856047 020__ $$a9783662546185$$q(electronic book)
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000856047 08204 $$a572.516$$223
000856047 1001_ $$aBi, Yunchen,$$eauthor.
000856047 24510 $$aStudy of the calcium regulation mechanism of TCR activation using nanodisc and NMR technologies /$$cYunchen Bi.
000856047 264_1 $$aBerlin, Germany :$$bSpringer,$$c[2017]
000856047 264_4 $$c©2017
000856047 300__ $$a1 online resource.
000856047 336__ $$atext$$btxt$$2rdacontent
000856047 337__ $$acomputer$$bc$$2rdamedia
000856047 338__ $$aonline resource$$bcr$$2rdacarrier
000856047 4901_ $$aSpringer theses : recognizing outstanding Ph. D. research,$$x2190-5053
000856047 504__ $$aIncludes bibliographical references.
000856047 5050_ $$6880-01$$aSupervisor's Foreword; Parts of the thesis have been published in the following journal articles:; Acknowledgements; Contents; 1 Introduction; Abstract; 1.1 Cell Membrane: Structure and Function; 1.1.1 Function of the Cell Membrane; 1.1.2 Composition of the Cell Membrane; 1.1.3 Structure of the Cell Membrane; 1.1.4 Phospholipid in Cell Membrane; 1.2 Membrane Proteins and in Vitro Reconstitution; 1.3 Membrane Binding Protein; 1.4 NMR Methods to Study Membrane Protein; 1.4.1 Background of NMR; 1.4.2 Use NMR to Study Protein Structure; 1.4.2.1 Protein Label and Sample Preparation.
000856047 5058_ $$a1.4.2.2 Collecting Data and Chemical Shift Assignment1.4.2.3 Obtaining the Constraints for Structure Calculation; 1.4.2.4 Structure Calculation and Refinement; 1.4.3 Using NMR to Study Protein Function; 1.4.3.1 Protein Dynamics Study by NMR; 1.4.3.2 NMR Studies of Protein-Ligand Interactions; 1.4.3.3 Studies of the Membrane Binding Protein (MBP) by 31P NMR; References; 2 Lipid Molecular-Ion Interaction Study Based on Nanodisc; Abstract; 2.1 What Is a Nanodisc?; 2.2 Optimizing Nanodisc Assembly for NMR Studies; 2.2.1 Experiment Methodology and Materials; 2.2.2 Results.
000856047 5058_ $$a3.2.7 Preparation of Nanodisc, Bicelles and Large Unilamellar Vesicles (LUVs)3.2.8 NMR Experiments; 3.2.9 Aromatic NOESY; 3.2.10 31P Spectrum; 3.3 Experiment Result; References; 4 Study on the Structure of Dengue Virus NS2B-NS3p in Complex with Aprotinin; Abstract; 4.1 Experiment Methods; 4.1.1 Clone, Expression and Purification of NS2B-NS3p; 4.1.2 NMR Sample Preparation; 4.1.3 NMR Spectra Collection and Data Processing; 4.2 Experiment Results; References; Conclusion and Future Work; Conclusion; Future Work; References.
000856047 506__ $$aAccess limited to authorized users.
000856047 588__ $$aOnline resource; title from PDF title page (viewed June 23, 2017).
000856047 650_0 $$aCalcium$$xMetabolism$$xRegulation.
000856047 650_0 $$aT cells$$xReceptors.
000856047 650_0 $$aNuclear magnetic resonance.
000856047 650_0 $$aCD antigens.
000856047 650_0 $$aDengue viruses.
000856047 77608 $$iPrint version:$$aBi, Yunchen.$$tStudy of the calcium regulation mechanism of TCR activation using nanodisc and NMR technologies.$$dBerlin, Germany : Springer, [2017]$$z3662546167$$z9783662546161$$w(OCoLC)975037554
000856047 830_0 $$aSpringer theses.
000856047 852__ $$bebk
000856047 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-662-54618-5$$zOnline Access$$91397441.1
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