Basic serological testing / Rowa Yousef Alhabbab.
Alhabbab, Rowa Yousef, author.
2018
RB46.5
Available Online

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Title Basic serological testing / Rowa Yousef Alhabbab.
Author Alhabbab, Rowa Yousef, author.
ISBN 9783319776941 (electronic book)
3319776940 (electronic book)
9783319776934
3319776932
DOI https://doi.org/10.1007/978-3-319-77694-1
Published Cham, Switzerland : Springer, [2018]
Language English
Description 1 online resource : illustrations
Other Standard Identifiers 10.1007/978-3-319-77694-1 doi
Call Number RB46.5
Dewey Decimal Classification 616.0756
Summary This book discusses in detail various serological tests that are used to identify medical conditions and diseases, from a general overview of each test to the equipment and steps needed to carry them out. The book is aimed specifically at immunology students and professionals who may occasionally need to use these tests, and thus lack training and experience in performing them. The book provides a brief overview of the immune system, including antibodies, antigens, and their interactions. The bulk of the book is comprised of 16 chapters that each explain different serological tests. These chapters start with a general introduction of the test or disease being detected, followed by the test principle, reagents required for the test, procedures and steps to perform the test, and, finally, result interpretation. Both test principles and result interpretation segments include illustrations to aid comprehension. In addition, the book also enables the reader to distinguish between positive and negative results in serological testing.
Bibliography, etc. Note Includes bibliographical references and index.
Access Note Access limited to authorized users.
Digital File Characteristics text file PDF
Source of Description Online resource; title from PDF title page (viewed May 30, 2018).
Series Techniques in life science and biomedicine for the non-expert.
Available in Other Form Basic serological testing.
Linked Resources Online Access
Record Appears in Online Resources > Ebooks
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Intro; Foreword; Preface; Acknowledgements; Contents; Chapter 1: Introduction to the Immune System; Innate Immunity; Physical and Chemical Barriers; Cells of the Innate Immune System; Complement; Adaptive Immunity; Clonal Selection Theory; Active, Passive, and Adoptive Immunization; Characteristics of the Adaptive Immune System; Cells Involved in the Adaptive Immune System; The Cellular and Humoral Adaptive Immune System; Humoral Immunity; Cell-Mediated Immunity; Study Questions; References; Chapter 2: Antibody and Antigen Interaction; Antigens; Antigen Foreignness; Antigen Molecular Weight.

Antigen Chemical ComplexityAntigen and MHC Molecules; Antibodies; Interaction of Antibodies and Antigens; Dilutions; C1V1 = C2V2 Method; Dilution Factor Method; Serial Dilution; General Safety Rules for Clinical Laboratories; Study Questions; References; Chapter 3: Precipitation and Agglutination Reactions; Precipitation Technique; Principle; Ring Precipitation Test; Application; Gel Diffusion Precipitation Test; Single Diffusion; Application; Double Diffusion; Application; Agglutination; Principle; Application; Direct Agglutination; Indirect Agglutination; Hemagglutination; Principle.

Direct HemagglutinationIndirect Hemagglutination; Application; Study Questions; References; Chapter 4: Rapid Plasma Reagin (RPR) Test; Rapid Plasma Reagin (RPR) Test; Principle; RPR Test Reagents; RPR Test Steps; Study Questions; References; Chapter 5: Treponema pallidum Hemagglutination (TPHA) Test; Treponema Pallidum Hemagglutination (TPHA) Test; Principle; Reagents That Must Be Provided in the Kit; TPHA Test Steps; Results Interpretation; Study Questions; References; Chapter 6: Stained Brucella Suspensions; The Most Pathogenic Brucella Species to Humans; Brucella abortus.

Brucella melitensisBrucella suis; Brucellosis Incubation Period; Serological Tests to Detect Brucellosis; Slide Agglutination Test; Principle; Reagents; Steps; Serum Agglutination Test, Tube (SAT); Principle; Reagents; SAT Test Steps; Results Interpretation; Results Example; Study Questions; References; Chapter 7: Rheumatoid Factor (RF); RF Latex Agglutination Test; Principle; The Application of the RF Latex Agglutination Test; Reagents and Materials Used; RF Latex Agglutination Test Steps; Results Interpretation; RF Latex Agglutination Test Limitations; Study Questions; References.

Chapter 8: Suspension Anti-Streptolysin-O (ASO/ASL) TestASO/ASL Test; Principle; ASO/ASL Test Application; ASO/ASL Test Reagents; ASO/ASL Test Steps; Results Interpretation; Study Questions; References; Chapter 9: C-Reactive Protein (CRP) Latex Agglutination Test; CRP Latex Agglutination Test; Principle; Applications and Significance; Reagents; Test Steps; Results Interpretation; Study Questions; References; Chapter 10: Complement Fixation Test (CFT); Complement Fixation Test; Principle; Reagents Provided in the Kit; Preparation of CFT Reagents.

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