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Intro; Preface; Acknowledgments; Contents; List of Contributors; About the Editor; Chapter 1: Principles of Atomic Force Microscopy; 1 AFM Working Principles; 2 Contact Mode; 3 Tapping Mode; 4 PID Feedback Loop; 5 Force Mode; 6 Peak Force Tapping; 7 Molecular Recognition; 8 Frequency Modulation Mode; Reference; Chapter 2: Atomic Force Microscopy-Based Single Molecule Force Spectroscopy for Biological Application; 1 Introduction; 1.1 AFM (Atomic Force Microscopy); 1.2 The Importance of Biomolecular Interactions; 2 Force Spectroscopy of Proteins on Cell Surface
3 Protein AFM-SMFS with DNA Aptamer Tips4 AFM-SMFS of Cell Surface Glycans; 5 Perspective; References; Chapter 3: In Situ Single Molecule Detection on Cell Membrane and Label Molecule Distributions Using AFM/NSOM; 1 Introduction; 2 Functionalization of AFM Tips; 3 Basic Principle for AFM-SMFS; 4 Basic Principle for AFM-TREC; 5 Basic Principle of NSOM for In Situ Single Molecule Detection on Cell Membrane; 6 Mapping Cell Membrane Biomolecules by AFM-SMFS; 7 Probing Cell Membrane Biomolecules by AFM-TREC; 8 Application of NSOM for In Situ Single Molecule Detection on Cell Membrane
9 Combining AFM and NSOM for In Situ Single Molecule Imaging on Cell Membrane10 Conclusions and Future Perspectives; References; Chapter 4: AFM Imaging-Force Spectroscopy Combination for Molecular Recognition at the Single-Cell Level; 1 Adhesion Force Mapping Using Force Spectroscopy; 2 Topographic and Recognition (TREC) Imaging; 3 Conclusions; References; Chapter 5: Atomic Force Microscopy: A Nanoscopic Application in Molecular and Cell Biology; 1 Introduction; 2 Basic Principles of AFM; 3 Submolecular Imaging of Cell Membranes; 4 Nanoscale Functional Imaging of Cells
4.1 Real-Time Imaging of Living Cells4.2 Imaging of Organelles and Proteins; 4.3 Multiparametric Force Probing and Molecular Recognition; 5 Combining Advanced Optical Microscopy with AFM; 5.1 Combining CLSM with AFM; 5.2 Combining NSOM with AFM; 6 Conclusions; References; Chapter 6: The Hyphenated Technique of High Speed Atomic Force Microscopy and Super Resolution Optical Detection System; 1 Introduction; 2 High Speed AFM; 2.1 A High Bandwidth Z-Scanner; 2.2 A Small Cantilever; 3 Combination of High Speed AFM with Super-Resolution Optical Techniques for Imaging; 3.1 High Speed AFM with STED
3.2 High Speed AFM with dSTORM3.3 High Speed AFM with PALM; 4 Hyphenated High Speed AFM and Super-Resolution Optical Techniques for Manipulation; 4.1 AFM Nanomanipulation; 4.2 Force Spectroscopy; 4.3 Fast Force Mapping; 4.4 AFM Manipulation - STED; 4.5 AFM Nano-Manipulation - Optical Spectroscopy; 4.6 AFM Manipulation - TIRF Microscopy; 5 Prospect and Outlook; 6 Conclusion; References; Chapter 7: AFM and NSOM/QD Based Direct Molecular Visualization at the Single-Cell Level; 1 AFM and NSOM/QD Based Nanoscale Imaging System
3 Protein AFM-SMFS with DNA Aptamer Tips4 AFM-SMFS of Cell Surface Glycans; 5 Perspective; References; Chapter 3: In Situ Single Molecule Detection on Cell Membrane and Label Molecule Distributions Using AFM/NSOM; 1 Introduction; 2 Functionalization of AFM Tips; 3 Basic Principle for AFM-SMFS; 4 Basic Principle for AFM-TREC; 5 Basic Principle of NSOM for In Situ Single Molecule Detection on Cell Membrane; 6 Mapping Cell Membrane Biomolecules by AFM-SMFS; 7 Probing Cell Membrane Biomolecules by AFM-TREC; 8 Application of NSOM for In Situ Single Molecule Detection on Cell Membrane
9 Combining AFM and NSOM for In Situ Single Molecule Imaging on Cell Membrane10 Conclusions and Future Perspectives; References; Chapter 4: AFM Imaging-Force Spectroscopy Combination for Molecular Recognition at the Single-Cell Level; 1 Adhesion Force Mapping Using Force Spectroscopy; 2 Topographic and Recognition (TREC) Imaging; 3 Conclusions; References; Chapter 5: Atomic Force Microscopy: A Nanoscopic Application in Molecular and Cell Biology; 1 Introduction; 2 Basic Principles of AFM; 3 Submolecular Imaging of Cell Membranes; 4 Nanoscale Functional Imaging of Cells
4.1 Real-Time Imaging of Living Cells4.2 Imaging of Organelles and Proteins; 4.3 Multiparametric Force Probing and Molecular Recognition; 5 Combining Advanced Optical Microscopy with AFM; 5.1 Combining CLSM with AFM; 5.2 Combining NSOM with AFM; 6 Conclusions; References; Chapter 6: The Hyphenated Technique of High Speed Atomic Force Microscopy and Super Resolution Optical Detection System; 1 Introduction; 2 High Speed AFM; 2.1 A High Bandwidth Z-Scanner; 2.2 A Small Cantilever; 3 Combination of High Speed AFM with Super-Resolution Optical Techniques for Imaging; 3.1 High Speed AFM with STED
3.2 High Speed AFM with dSTORM3.3 High Speed AFM with PALM; 4 Hyphenated High Speed AFM and Super-Resolution Optical Techniques for Manipulation; 4.1 AFM Nanomanipulation; 4.2 Force Spectroscopy; 4.3 Fast Force Mapping; 4.4 AFM Manipulation - STED; 4.5 AFM Nano-Manipulation - Optical Spectroscopy; 4.6 AFM Manipulation - TIRF Microscopy; 5 Prospect and Outlook; 6 Conclusion; References; Chapter 7: AFM and NSOM/QD Based Direct Molecular Visualization at the Single-Cell Level; 1 AFM and NSOM/QD Based Nanoscale Imaging System