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Table of Contents
Intro; Preface; Acknowledgments; Contents; Part I: Regenerative Medicine and the Role of Tissue Engineering in Maxillomandibular Reconstruction; 1: Principles of Regenerative Medicine of the Maxillomandibular Region; 1.1 Considerations in Maxillomandibular Reconstruction; 1.2 Tissue Transfer; 1.3 Tissue Engineering and Regenerative Medicine; 1.4 Biomaterial Scaffolds and Extracellular Matrix; 1.5 Cells; 1.6 Biologically Active Factors and the Extracellular Environment; 1.7 Current and Future Applications in Maxillofacial Reconstruction; References
2: Traditional Use of Autogenous and Non-autogenous Grafts in Head and Neck Reconstruction: Principles of Conventional Bone Grafting2.1 Autogenous Versus Non-autogenous Bone Grafts; 2.2 Conventional Autogenous Non-vascularized Grafts; 2.2.1 Anterior and Posterior Iliac Crest; 2.2.2 Costochondral Graft; 2.2.3 Tibial Metaphysis Graft; 2.2.4 Calvarial Graft; 2.2.5 Oral Bone Grafts; 2.3 Conclusion; References; 3: Reconstruction Considerations for the Posttraumatic, Benign Tumor, and Oncologic Patient; 3.1 Introduction; 3.2 Reconstructive Options; 3.3 Posttraumatic
3.4 Malignant Tumors3.5 Benign Tumors; 3.6 Conclusion; References; 4: The Role of rhBMP-2 in Oral and Maxillofacial Reconstruction; 4.1 Introduction; 4.2 What Are They?; 4.3 How Do They Work?; 4.4 Clinically What Does It Look Like and How Do I Use It?; 4.5 Does It Work? What Can I Use It For?; 4.6 Combination with Autografts; 4.7 Limits, Adverse Effects, and Contraindications with rhBMP-2/ACS; 4.8 Conclusion; References; 5: Techniques of Obtaining BMAC, PRP, and PRF; 5.1 PRF Preparation; 5.1.1 PRF Membrane Fabrication; 5.1.2 PRF Bone Putty; 5.2 PRF Applications
5.2.1 PRF in Particulate Grafting Procedures5.2.2 PRF Membrane as a Palatal Bandage for Free Gingival Graft (FGG) Donor Site; Reference; 6: Principles of Soft Tissue Engineering for Craniomaxillofacial Reconstruction; 6.1 Introduction; 6.2 Proposed Clinical Applications for Reconstruction of the Lips; 6.2.1 Advantages of Our TE/RM Approach; 6.2.2 Proposed Study Design; 6.2.3 Challenges and Potential Problems; 6.3 Materials and Reagents; 6.3.1 Materials; 6.3.2 Reagents; 6.4 Methods; 6.4.1 Keratinocytes Culture; 6.4.1.1 Oral Keratinocytes Culture; 6.4.1.2 Skin Keratinocyte Culture
6.4.2 3D Organotypic Culture Using EVPOMEs (Ex Vivo Produced Oral Mucosa Equivalents) to Manufacture Soft Tissue Engineering Devices6.4.2.1 EVPOME Using Single Cell Type, Oral Keratinocytes, Culture; 6.4.2.2 EVPOME Using Floating Keratinocytes Culture; 6.4.2.3 EVPOME Using Two Cell Types Co-cultures; Making of Barrier, Corral, and Waffle; Manufacturing EVPOME Using Oral and Skin Keratinocytes Co-cultures; 6.5 Notes; References; Part II: Surgical Techniques Utilizing Biotechnology for Regeneration and Reconstruction; 7: In Situ Tissue Engineering; 7.1 In Situ Bone Tissue Engineering
2: Traditional Use of Autogenous and Non-autogenous Grafts in Head and Neck Reconstruction: Principles of Conventional Bone Grafting2.1 Autogenous Versus Non-autogenous Bone Grafts; 2.2 Conventional Autogenous Non-vascularized Grafts; 2.2.1 Anterior and Posterior Iliac Crest; 2.2.2 Costochondral Graft; 2.2.3 Tibial Metaphysis Graft; 2.2.4 Calvarial Graft; 2.2.5 Oral Bone Grafts; 2.3 Conclusion; References; 3: Reconstruction Considerations for the Posttraumatic, Benign Tumor, and Oncologic Patient; 3.1 Introduction; 3.2 Reconstructive Options; 3.3 Posttraumatic
3.4 Malignant Tumors3.5 Benign Tumors; 3.6 Conclusion; References; 4: The Role of rhBMP-2 in Oral and Maxillofacial Reconstruction; 4.1 Introduction; 4.2 What Are They?; 4.3 How Do They Work?; 4.4 Clinically What Does It Look Like and How Do I Use It?; 4.5 Does It Work? What Can I Use It For?; 4.6 Combination with Autografts; 4.7 Limits, Adverse Effects, and Contraindications with rhBMP-2/ACS; 4.8 Conclusion; References; 5: Techniques of Obtaining BMAC, PRP, and PRF; 5.1 PRF Preparation; 5.1.1 PRF Membrane Fabrication; 5.1.2 PRF Bone Putty; 5.2 PRF Applications
5.2.1 PRF in Particulate Grafting Procedures5.2.2 PRF Membrane as a Palatal Bandage for Free Gingival Graft (FGG) Donor Site; Reference; 6: Principles of Soft Tissue Engineering for Craniomaxillofacial Reconstruction; 6.1 Introduction; 6.2 Proposed Clinical Applications for Reconstruction of the Lips; 6.2.1 Advantages of Our TE/RM Approach; 6.2.2 Proposed Study Design; 6.2.3 Challenges and Potential Problems; 6.3 Materials and Reagents; 6.3.1 Materials; 6.3.2 Reagents; 6.4 Methods; 6.4.1 Keratinocytes Culture; 6.4.1.1 Oral Keratinocytes Culture; 6.4.1.2 Skin Keratinocyte Culture
6.4.2 3D Organotypic Culture Using EVPOMEs (Ex Vivo Produced Oral Mucosa Equivalents) to Manufacture Soft Tissue Engineering Devices6.4.2.1 EVPOME Using Single Cell Type, Oral Keratinocytes, Culture; 6.4.2.2 EVPOME Using Floating Keratinocytes Culture; 6.4.2.3 EVPOME Using Two Cell Types Co-cultures; Making of Barrier, Corral, and Waffle; Manufacturing EVPOME Using Oral and Skin Keratinocytes Co-cultures; 6.5 Notes; References; Part II: Surgical Techniques Utilizing Biotechnology for Regeneration and Reconstruction; 7: In Situ Tissue Engineering; 7.1 In Situ Bone Tissue Engineering