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Intro; Foreword; Preface; Acknowledgements; Contents; Contributors; Abbreviations; Introduction of Nucleic Acid Amplification Strategies; 1 Nucleic Acid Amplification Strategies for Biosensing, Bioimaging, and Biomedicine; 1.1 General; 1.2 The Typical Nucleic Acid Amplification Strategies; 1.2.1 Thermocycling Amplification Techniques; 1.2.2 Isothermal Amplification Techniques; 1.3 Overview of This Book; References; Nucleic Acid Amplification Strategies for Biosensing; 2 Fluorescence Techniques Based on Nucleic Acid Amplification Strategies: Rational Design and Application; 2.1 Introduction
2.2 Fluorescent Dyes and Nanomaterials2.2.1 Organic Fluorescent Dyes; 2.2.2 Quantum Dots (QDs); 2.2.3 Metal Nanoclusters (MNCs); 2.2.4 Upconversion Nanomaterials; 2.2.5 Cationic Conjugated Polymers; 2.3 Fluorescence Reporter Based on Nucleic Acid Amplification Strategies; 2.3.1 Fluorescent Dyes Aggression; 2.3.2 Fluorescence Resonance Energy Transfer (FRET); 2.3.3 Photoinduced Electron Transfer Strategy; 2.4 Rational Design of Fluorescence Biosensor Based on Nucleic Acid Amplification Strategies; 2.4.1 Fluorophores Chemically Conjugated to DNA; 2.4.2 A Label-Free Fluorescent Strategy
2.5 Fluorescence Collection and Commercial Instrument2.5.1 Real-Time or Quantitative Nucleic Acid Amplification Strategies; 2.5.2 Multiplex Nucleic Acid Amplification Strategies; 2.5.3 Digital Nucleic Acid Amplification Strategies; 2.6 Perspectives; References; 3 Nucleic Acid Amplification Strategies-Based Chemiluminescence Biosensors; 3.1 Nucleic Acid-Based CL Nanoprobes for Signal Amplification; 3.2 Development of CL Systems for Signal Amplification; 3.3 Tool Enzyme Amplification-Based CL Biosensors; 3.4 Enzyme-Free Signal Amplification CL Biosensors; References
4 Nucleic Acid Amplification Strategy-Based Electrochemiluminescence Research4.1 General; 4.1.1 Electrochemiluminescence (ECL) Assays; 4.2 Nucleic-Acid-Amplification-Assisted ECL Assays; 4.2.1 Nucleic Acid-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.2.2 Proteins-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.2.3 Cancer Cells-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.2.4 Small Molecules-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.3 Conclusions and Perspectives; References
5 Nucleic Acid Amplification Strategy-Based Colorimetric Assays5.1 Introduction; 5.2 Colorimetric Assays Based on Au Nanoparticles; 5.2.1 Colorimetric Assays Detection Based on Enzyme-Assisted Strategy Amplification and AuNPs; 5.2.2 Colorimetric Assays Detection Based on Enzymes-Free Strategies Amplification and AuNPs; 5.3 Colorimetric Assay Based on Catalyzation; 5.3.1 Colorimetric Assay Based on HRP Catalyst; 5.3.2 Colorimetric Assay Based on Mimic HRP Catalyst; 5.4 Colorimetric Assays Based on Other Indicators; 5.5 Conclusion; References
2.2 Fluorescent Dyes and Nanomaterials2.2.1 Organic Fluorescent Dyes; 2.2.2 Quantum Dots (QDs); 2.2.3 Metal Nanoclusters (MNCs); 2.2.4 Upconversion Nanomaterials; 2.2.5 Cationic Conjugated Polymers; 2.3 Fluorescence Reporter Based on Nucleic Acid Amplification Strategies; 2.3.1 Fluorescent Dyes Aggression; 2.3.2 Fluorescence Resonance Energy Transfer (FRET); 2.3.3 Photoinduced Electron Transfer Strategy; 2.4 Rational Design of Fluorescence Biosensor Based on Nucleic Acid Amplification Strategies; 2.4.1 Fluorophores Chemically Conjugated to DNA; 2.4.2 A Label-Free Fluorescent Strategy
2.5 Fluorescence Collection and Commercial Instrument2.5.1 Real-Time or Quantitative Nucleic Acid Amplification Strategies; 2.5.2 Multiplex Nucleic Acid Amplification Strategies; 2.5.3 Digital Nucleic Acid Amplification Strategies; 2.6 Perspectives; References; 3 Nucleic Acid Amplification Strategies-Based Chemiluminescence Biosensors; 3.1 Nucleic Acid-Based CL Nanoprobes for Signal Amplification; 3.2 Development of CL Systems for Signal Amplification; 3.3 Tool Enzyme Amplification-Based CL Biosensors; 3.4 Enzyme-Free Signal Amplification CL Biosensors; References
4 Nucleic Acid Amplification Strategy-Based Electrochemiluminescence Research4.1 General; 4.1.1 Electrochemiluminescence (ECL) Assays; 4.2 Nucleic-Acid-Amplification-Assisted ECL Assays; 4.2.1 Nucleic Acid-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.2.2 Proteins-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.2.3 Cancer Cells-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.2.4 Small Molecules-ECL Biosensors Assisted by Nucleic Acid Amplification Strategies; 4.3 Conclusions and Perspectives; References
5 Nucleic Acid Amplification Strategy-Based Colorimetric Assays5.1 Introduction; 5.2 Colorimetric Assays Based on Au Nanoparticles; 5.2.1 Colorimetric Assays Detection Based on Enzyme-Assisted Strategy Amplification and AuNPs; 5.2.2 Colorimetric Assays Detection Based on Enzymes-Free Strategies Amplification and AuNPs; 5.3 Colorimetric Assay Based on Catalyzation; 5.3.1 Colorimetric Assay Based on HRP Catalyst; 5.3.2 Colorimetric Assay Based on Mimic HRP Catalyst; 5.4 Colorimetric Assays Based on Other Indicators; 5.5 Conclusion; References