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Machine generated contents note: 1. Once upon a (length and) time (scale)...; 2. The molecules of life; 3. Making the invisible visible: part 1; 4. Making the invisible visible: part 2; 5. Measuring forces and manipulating single molecules; 6. Single molecule biophysics; 7. Molecules from beyond; 8. Into the membrane; 9. Inside cells; 10. Single molecule biophysics beyond the single cell and beyond the single molecule; Index.