000914814 000__ 04530cam\a2200493Ia\4500 000914814 001__ 914814 000914814 005__ 20230306150544.0 000914814 006__ m\\\\\o\\d\\\\\\\\ 000914814 007__ cr\un\nnnunnun 000914814 008__ 190926s2019\\\\sz\\\\\\o\\\\\000\0\eng\d 000914814 020__ $$a9783030282479$$q(electronic book) 000914814 020__ $$a3030282473$$q(electronic book) 000914814 020__ $$z3030282465 000914814 020__ $$z9783030282462 000914814 035__ $$aSP(OCoLC)on1120984860 000914814 035__ $$aSP(OCoLC)1120984860 000914814 040__ $$aYDX$$beng$$cYDX$$dGW5XE$$dEBLCP 000914814 049__ $$aISEA 000914814 050_4 $$aQH652 000914814 08204 $$a571.4/5$$223 000914814 24500 $$aRadiation in bioanalysis :$$bspectroscopic techniques and theoretical methods /$$cAlice S. Pereira, Pedro Tavares, Paulo Limão-Vieira, editors. 000914814 260__ $$aCham :$$bSpringer,$$c2019. 000914814 300__ $$a1 online resource 000914814 336__ $$atext$$btxt$$2rdacontent 000914814 337__ $$acomputer$$bc$$2rdamedia 000914814 338__ $$aonline resource$$bcr$$2rdacarrier 000914814 4901_ $$aBioanalysis, Advanced Materials, Methods, and Devices,$$x2364-1118 ;$$vv .8 000914814 5050_ $$aIntro; Editorial; Contents; Spectroscopic and Analyses Techniques; 1 High-Resolution Electron Energy Loss Spectroscopy: Absolute Cross Section Measurements for Low Energy Electron Scattering from Biomolecules; 1.1 Introduction; 1.1.1 General; 1.1.2 Radiotherapy Modalities and Low-Energy Electrons; 1.1.3 Monte Carlo Simulations and Nanodosimetry; 1.2 Absolute Cross Sections Measurements with HREELS; 1.2.1 Condensed Phase Scattering; 1.2.2 HREEL Spectroscopy; 1.2.3 Absolute Cross Sections for Condensed Biomolecules; 1.3 Nanodosimetry Using Absolute Cross Sections from HREELS Measurements 000914814 5058_ $$a1.4 Future Trends1.5 Conclusion; References; 2 Synchrotron Radiation UV-VUV Photoabsorption of Gas Phase Molecules; 2.1 Introduction; 2.2 Photoabsorption Basics; 2.2.1 The Beer Lambert Law; 2.2.2 Cross-Section; 2.2.3 Absorption of Light and the Dipole Approximation; 2.2.4 Valence and Rydberg States; 2.2.5 Coupling to Photoelectron Spectroscopy (PES); 2.2.6 Comparison to Electron Energy Loss Spectroscopy; 2.3 Experimental Set-Up; 2.3.1 A Source of VUV-Visible Light; 2.3.2 The AU-UV Beam Line; 2.3.3 The Gas Phase Photoabsorption Apparatus 000914814 5058_ $$a2.3.4 Subtraction Methods for Rydberg and Vibrational State Analysis2.3.5 Other SR Photo-absorption Beam Lines (Overview); 2.4 Electronic Structure Methods; 2.4.1 Short Introduction to Ab Initio Methods; 2.4.2 Atomic Basis Sets; 2.4.3 Assignment of Experimental Spectra; 2.4.4 Photoelectron Spectra; 2.4.5 Vibrational Fine Structure; 2.5 Investigated Classes of Molecules; 2.5.1 An Example of a Series of Photoabsorption Spectra: Substituted Benzenes; 2.6 Outlook; References; 3 Fluorescence Spectroscopy; 3.1 General Considerations; 3.1.1 Excited State Deactivation Pathways for Molecules 000914814 5058_ $$a3.1.2 Quantum Yields3.1.3 Excited State Lifetimes; 3.1.4 Stern-Volmer Analysis and Quenching Mechanisms; 3.1.5 Concentration Quenching and Excimer Formation; 3.1.6 Charge Transfer Mechanism: Exciplex Formation and Decay; 3.1.7 Energy Transfer Mechanisms; 3.2 Experimental Aspects of Fluorescence Spectroscopy; 3.2.1 The Spectrofluorometer; 3.2.2 Acquisition of Spectra; 3.2.3 Troubleshooting; 3.2.4 Fluorescence Versus Phosphorescence; 3.2.5 Low Temperature Luminescence; References; 4 Raman Biospectroscopy and Imaging; 4.1 Introduction; 4.2 Methodology; 4.2.1 Raman Spectroscopy (RS) 000914814 5058_ $$a4.2.2 Advanced Raman Spectroscopy4.2.3 Raman Instrument; 4.3 Applications; 4.3.1 Raman Spectroscopy for Bioanalysis; 4.3.2 Resonance Raman Spectroscopy of Biomolecules Containing Metal or Organic Chromophores; 4.3.3 Surface Enhanced (Resonance) Raman Spectroscopy of Biomolecules; 4.3.4 Raman Imaging of Cells and Tissues; 4.4 Outlook; References; 5 Circular Dichroism and Synchrotron Radiation Circular Dichroism Applications to Biomaterials; 5.1 Introduction; 5.2 B23 Beamline for Synchrotron Radiation Circular Dichroism (SRCD); 5.2.1 B23 Higher Photon Flux; 5.2.2 Protein UV-Denaturation Assay 000914814 506__ $$aAccess limited to authorized users. 000914814 650_0 $$aRadioisotopes in biology. 000914814 650_0 $$aRadiobiology. 000914814 7001_ $$aPereira, Alice S. 000914814 7001_ $$aTavares, Pedro. 000914814 7001_ $$aLimão-Vieira, Paulo. 000914814 77608 $$iPrint version: $$z3030282465$$z9783030282462$$w(OCoLC)1107593380 000914814 830_0 $$aBioanalysis (Springer (Firm)) ;$$vv .8. 000914814 852__ $$bebk 000914814 85640 $$3SpringerLink$$uhttps://univsouthin.idm.oclc.org/login?url=http://link.springer.com/10.1007/978-3-030-28247-9$$zOnline Access$$91397441.1 000914814 909CO $$ooai:library.usi.edu:914814$$pGLOBAL_SET 000914814 980__ $$aEBOOK 000914814 980__ $$aBIB 000914814 982__ $$aEbook 000914814 983__ $$aOnline 000914814 994__ $$a92$$bISE