Linked e-resources
Details
Table of Contents
Intro
Foreword
Preface
Contents
Part I: Current Status and Challenges of Plant Genome Editing Using CRISPR/Cas Technology
Genome Engineering as a Tool for Enhancing Crop Traits: Lessons from CRISPR/Cas9
1 Introduction
2 Zinc-Finger Nucleases
3 Transcription Activator-Like Effector Nucleases
4 CRISPR/Cas9 System
5 Mechanism of CRISPR/Cas9 System
6 Targeted Improvement of Crop Traits Using CRISPR/Cas9-Based Genome Editing in Cereals
6.1 Resistance Against Bacterial Disease
6.2 Resistance Against Fungal Disease
6.3 Resistance Against Viruses
6.4 Resistance and Tolerance Against Herbicides
6.5 Improved Quality and Yield
7 CRISPR/Cas9 Mediated Genome Editing in Horticultural Crops
7.1 Resistance Against Bacterial Disease
7.2 Resistance Against Fungal Disease
7.3 Resistance Against Viruses
7.4 Resistance and Tolerance Against Herbicide
7.5 Improved Quality and Yield
8 Conclusion
References
Vegetable Crop Improvement Through CRISPR Technology for Food Security
1 Introduction
2 Applications of Genome Editing in Improvement of Vegetable Crops
2.1 Qualitative Traits
2.1.1 Starch Content
2.1.2 Pigmentation
2.1.3 Saturated Fatty Acid Content
2.1.4 Improvement of Shelf-Life and Quality
2.1.5 Other Qualitative Traits
2.2 Abiotic Stress Tolerance
2.2.1 Drought and Extreme Temperature
2.2.2 Salinity and Mineral Deficiency
2.3 Pest and Disease Resistance
2.4 Biosafety and Legal Regulations
2.5 Conclusion
References
CRISPR/Cas9-Mediated Targeted Mutagenesis in Medicinal Plants
1 Introduction
2 CRISPR/Cas9 Mechanism
2.1 Cleavage Activity of Cas9
3 CRISPR/Cas9 Vector System for Plants
3.1 sgRNA Expression Cassettes
3.2 Cas9 Expression Cassettes
4 CRISPR/dCas9 and Epigenome Editing in Plants
4.1 Nuclease-Dead Cas9
4.2 sgRNA
4.3 Transcriptional Effectors
5 Analysis and Efficiency of Targeted Mutations
5.1 Reporter Genes
5.2 Single-Strand Conformation Polymorphism (SSCP)
5.3 High-Resolution Melting (HRM)
5.4 High-Throughput Sequencing (HTS or Deep Sequencing)
5.5 Sanger Sequencing
6 Medicinal Plants Modified Using CRISPR/Cas9
6.1 Salvia militorrhiza
6.2 Dendrobium officinale
6.3 Dioscorea zingiberensis
7 Applications of Genome Editing in Medicinal Plants
8 Conclusion
References
Genome Editing: A Review of the Challenges and Approaches
1 Introduction
2 Mechanisms of Repairing Double-Stranded Breaks
2.1 Non-Homologous End Joining (NHEJ)
2.2 Genome editing and Homologous Recombination
2.2.1 History of Genome Editing
2.2.2 Homologous Recombination in E. coli
2.2.3 HR in Saccharomyces cerevisiae
2.2.4 HR in Higher Organisms
3 Different Genome Editing Techniques
3.1 Meganucleases (MNs)
3.2 Zinc Finger Nucleases (ZFNs)
3.3 Transcription Activator-Like Effector Nucleases (TALENs)
Foreword
Preface
Contents
Part I: Current Status and Challenges of Plant Genome Editing Using CRISPR/Cas Technology
Genome Engineering as a Tool for Enhancing Crop Traits: Lessons from CRISPR/Cas9
1 Introduction
2 Zinc-Finger Nucleases
3 Transcription Activator-Like Effector Nucleases
4 CRISPR/Cas9 System
5 Mechanism of CRISPR/Cas9 System
6 Targeted Improvement of Crop Traits Using CRISPR/Cas9-Based Genome Editing in Cereals
6.1 Resistance Against Bacterial Disease
6.2 Resistance Against Fungal Disease
6.3 Resistance Against Viruses
6.4 Resistance and Tolerance Against Herbicides
6.5 Improved Quality and Yield
7 CRISPR/Cas9 Mediated Genome Editing in Horticultural Crops
7.1 Resistance Against Bacterial Disease
7.2 Resistance Against Fungal Disease
7.3 Resistance Against Viruses
7.4 Resistance and Tolerance Against Herbicide
7.5 Improved Quality and Yield
8 Conclusion
References
Vegetable Crop Improvement Through CRISPR Technology for Food Security
1 Introduction
2 Applications of Genome Editing in Improvement of Vegetable Crops
2.1 Qualitative Traits
2.1.1 Starch Content
2.1.2 Pigmentation
2.1.3 Saturated Fatty Acid Content
2.1.4 Improvement of Shelf-Life and Quality
2.1.5 Other Qualitative Traits
2.2 Abiotic Stress Tolerance
2.2.1 Drought and Extreme Temperature
2.2.2 Salinity and Mineral Deficiency
2.3 Pest and Disease Resistance
2.4 Biosafety and Legal Regulations
2.5 Conclusion
References
CRISPR/Cas9-Mediated Targeted Mutagenesis in Medicinal Plants
1 Introduction
2 CRISPR/Cas9 Mechanism
2.1 Cleavage Activity of Cas9
3 CRISPR/Cas9 Vector System for Plants
3.1 sgRNA Expression Cassettes
3.2 Cas9 Expression Cassettes
4 CRISPR/dCas9 and Epigenome Editing in Plants
4.1 Nuclease-Dead Cas9
4.2 sgRNA
4.3 Transcriptional Effectors
5 Analysis and Efficiency of Targeted Mutations
5.1 Reporter Genes
5.2 Single-Strand Conformation Polymorphism (SSCP)
5.3 High-Resolution Melting (HRM)
5.4 High-Throughput Sequencing (HTS or Deep Sequencing)
5.5 Sanger Sequencing
6 Medicinal Plants Modified Using CRISPR/Cas9
6.1 Salvia militorrhiza
6.2 Dendrobium officinale
6.3 Dioscorea zingiberensis
7 Applications of Genome Editing in Medicinal Plants
8 Conclusion
References
Genome Editing: A Review of the Challenges and Approaches
1 Introduction
2 Mechanisms of Repairing Double-Stranded Breaks
2.1 Non-Homologous End Joining (NHEJ)
2.2 Genome editing and Homologous Recombination
2.2.1 History of Genome Editing
2.2.2 Homologous Recombination in E. coli
2.2.3 HR in Saccharomyces cerevisiae
2.2.4 HR in Higher Organisms
3 Different Genome Editing Techniques
3.1 Meganucleases (MNs)
3.2 Zinc Finger Nucleases (ZFNs)
3.3 Transcription Activator-Like Effector Nucleases (TALENs)