1. Overview to animal cell culture
2. Cell culture laboratory
3. Good lab practices and bio-safety measures
4. Managing sterility in the lab
5. Media and buffer preparation for cell culture
6. Properties of cultured cells and selection of culture media
7. Selection and maintenance of cultured cells
8. Inoculation and passaging of adherent cells
9. Counting of cells
10. Cryopreservation of cell lines
11. Resuscitation of frozen cell line
12. Isolation and culturing of cells from different tissues
13. Differentiation, transformation and immortalization of cells
14. Co-culture techniques
15. 3d cell culture techniques
16. Stem cell culture techniques
17. Identification and removal of biological contamination in the media and cell suspensions
18. Analysis of cell growth kinetics in suspension and adherent types cell line
19. Applications of animal cell culture based assays
20. Ethical issues in animal cell culture
21. Common troubleshooting methods in cell culture techniques.