CPP, cell-penetrating peptides [electronic resource] / Ülo Langel.
Langel, Ülo, author.
2023
RS199.5 .L36 2023
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Title CPP, cell-penetrating peptides [electronic resource] / Ülo Langel.
Author Langel, Ülo, author.
Edition Second edition.
ISBN 9783031387319
3031387317
DOI https://doi.org/10.1007/978-3-031-38731-9
Imprint Cham, Switzerland : Springer, [2023]
Language English
Description 1 online resource (xxi, 556) ; illustrations
Other Standard Identifiers 10.1007/978-3-031-38731-9 doi
Call Number RS199.5 .L36 2023
Dewey Decimal Classification 572/.65
Summary In this book, a summary and update of the most important areas of cell-penetrating peptides (CPP) research are presented, while raising relevant questions for further development. The CPP sequences are presented and discussed throughout the book. The methods for testing CPP mechanisms are discussed in detail. Various approaches for the testing of endocytotic pathways of CPP uptake are also described. Different CPP uptake experiments are compared since it is becoming clear that it is often best to apply several methods in a complementary manner in order to most comprehensively evaluate CPP uptake mechanisms due to the complexity of these processes. A brief summary of functionality issues of CPPs, both in vitro and in vivo, is discussed. Therapeutic potential of CPPs and commercial developments are discussed. The present, second edition of this book is the updated and expanded version of the first edition, published in 2019. The development of the field of cell-penetrating peptides in these five years has been obvious and exciting. This second edition of the book has been partly reorganized and comprehensively expanded with the exciting research in 2019-2023. Around 2500 novel scientific articles have become available, most of them are reviewed in the second edition. Additional rapidly growing areas of high impact presented in this second edition are therapeutic developments (Chapter 16) and delivery of oligonucleotides and proteins/peptides (Chapters 5 and 6) including novel reports on genome editing with CPP assistance. Also, several additional examples are available now on clinical trials using CPPs (Chapter 15). The book is written for researchers and students in the field.
Note Description based upon print version of record.
10.4.1 Applications of Fluorophores in Imaging
Bibliography, etc. Note Includes bibliographical references and index.
Access Note Access limited to authorized users.
Available in Other Form CPP, Cell-Penetrating Peptides
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Record Appears in Online Resources > Ebooks
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Intro
Acknowledgment
Contents
Abbreviations
About the Author
List of Figures
Contents
1 Introduction
1.1 Introduction to the Second Edition, 2024
1.2 Introduction to First Edition
References
2 Classes and Applications of Cell-Penetrating Peptides
Abstract
2.1 Protein Derived Versus Designed
2.2 Classification by Physico-Chemical Properties Versus Structural Properties
2.3 Predicted Versus Random
2.4 Linear Versus Modified
2.4.1 Linear
2.4.2 Stabilized
2.5 Protein Mimics Versus Cargo Delivery Vectors
2.5.1 Protein-Mimicking CPPs

2.5.2 CPPs for Cargo Delivery
2.6 Nonspecific Versus Targeted
2.6.1 Affinity Targeting: Homing, Ligand Based Targeting
2.6.2 Prodrug Approach, aCPP
2.7 Classification by Uptake Mechanisms
2.7.1 Direct Translocators
2.7.2 Endocytosis Enhancers
2.8 Non-toxic Versus Antimicrobial
2.8.1 Non-toxic
2.8.2 Antimicrobial
References
3 Methods for CPP Selection, Prediction and in Silico Analysis
Abstract
3.1 Selection
3.2 Prediction
3.3 Cargo Delivery Vectors
References
4 Labeling of CPPs
Abstract
4.1 Radioactive CPP Labelling

4.2 Fluorescent Labelling of CPPs
4.3 Quantum Dots
4.4 Fluorescence Quenching
4.5 Luciferin-CPP Conjugates
References
5 Methods for CPP Functionalization with Oligonucleotides
Abstract
5.1 Antisense
5.1.1 Antisense Delivery
5.1.2 Splicing Redirection Assay
5.1.3 MicroRNAs and Anti-MicroRNAs
5.1.4 Decoy Oligonucleotides
5.1.5 Live Cell Genomics
5.2 siRNA Delivery Methods
References
6 Additional Gene Therapeutic Platforms
Abstract
6.1 Plasmid Delivery
6.2 Cellular Reprogramming
6.3 Genome Editing
References

7 Protein Delivery and Mimicry
Abstract
7.1 Fusion Proteins
7.2 Chemical Conjugation of CPPs to Proteins
7.3 Biotin Conjugations
7.4 Complexation of Proteins and Peptides
7.5 Targeting Proteins with CPPs
7.6 Protein Mimicry and PPI
7.6.1 Delivery of Protein Mimics as Cargos by CPPs
7.6.2 Protein Mimics with CPP Properties
References
8 General Targeting Strategies
Abstract
8.1 "Addressing"
8.2 Prodrug Strategies
References
9 Targeting Specific Barriers
Abstract
9.1 Targeting Intracellular Organelles with CPPs

9.1.1 Nuclear and Nucleolar Targeting
9.1.2 Lysosomal Targeting
9.1.3 Mitochondrial Targeting
9.1.4 Golgi and Endoplasmic Reticulum Targeting
9.2 Targeting Biological Tissue Barriers
9.2.1 Oral Administration
9.2.2 Inhalation Delivery
9.2.3 Intranasal Delivery
9.2.4 Transdermal Delivery
9.2.5 Delivery Across Blood-brain-Barrier, BBB
9.3 Targeting Plants
References
10 Methods for Molecular Imaging, Detection and Visualization of CPPs
Abstract
10.1 HPLC Analysis
10.2 Mass-Spectrometric Methods
10.3 Radionuclide Imaging
10.4 Optical Imaging

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