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Table of Contents
Intro
Foreword
Preface
Acknowledgements
Contents
Editor and Contributors
1: Recent Developments in Biosensor Technology for Fisheries and Aquaculture
1.1 Introduction
1.2 Working Principle of Biosensor
1.3 Main Components of Biosensor
1.4 Types of Biosensors
1.4.1 Electrochemical Biosensor
1.4.1.1 Amperometric Biosensor
1.4.1.2 Potentiometric Biosensor
1.4.1.3 Impedimetric Biosensor
1.4.1.4 Voltammetric Biosensor
1.4.2 Physical Biosensor
1.4.2.1 Piezoelectric Biosensors
1.4.2.2 Thermometric Biosensor
1.4.3 Optical Biosensor
1.4.4 Wearable Biosensors
1.4.5 Enzyme Biosensor
1.4.6 DNA Biosensor
1.4.7 Immunosensors
1.4.7.1 Magnetic Biosensors
1.4.7.2 Resonant Biosensors
1.4.7.3 Thermal Detection Biosensor
1.4.8 Biosensors in Aquaculture
1.4.8.1 Ammonium Detection in Aquaculture
1.4.8.2 Detection of Water Pollutants
1.4.9 Biosensor for Fish Health Monitoring
1.4.9.1 Biosensor for VHSV Detection
1.4.9.2 Biosensor for Aphanomyces invadans
1.4.9.3 Aptamer-Based Biosensors for Pathogen Detection
1.4.9.4 Vibrio parahemolyticus
1.4.9.5 Vibrio vulnificus
1.4.9.6 Vibrio alginolyticus
1.4.9.7 Vibrio harveyi
1.4.9.8 Listeria monocytogenes
1.5 Conclusion
References
2: Application of CRISPR-Cas9 Technology in Fish
2.1 Introduction
2.2 Genome Editing Platforms
2.2.1 Zinc Finger Nuclease
2.2.2 Transcription Activator-Like Effector Nuclease
2.2.3 Clustered Regularly Interspersed Short Palindromic Sequence
2.2.3.1 Classification of CRISPR/Cas System
2.3 Genome Editing Using CRISPR/Cas9 System
2.3.1 Selection of Target Gene
2.3.2 Designing of gRNA
2.3.3 Delivery of sgRNA Complex
2.3.4 Production and Maintenance of Homozygous Mutant
2.4 Application of CRISPR/Cas9 in Fisheries and Aquaculture
2.4.1 Improvement of Aquaculture Important Traits
2.4.1.1 Somatic Growth and Production
2.4.2 Disease Resistance and Stress Tolerance
2.4.3 Sterility and Reproductive Confinement
2.4.4 Flesh Quality and Omega-3 Metabolism
2.4.5 Pigmentation
2.4.6 Application in Disease Diagnosis
2.4.7 Application in Functional Characterisation of Genes
2.4.8 Other Aquaculture Important Applications
2.5 Challenges in Application of GE-Based Tools in Fisheries and Aquaculture
2.5.1 Lack of Robust Knowledge of Teleost Genomes
2.5.2 Non-availability of Suitable Cell Lines
2.5.3 Development of Standardised Species-Specific Method
2.5.4 Effect of Whole Genome Duplication
2.5.5 Public Acceptance and Regulations
2.6 Commercialisation
2.7 Future Aspects and Conclusion
References
3: Nanotechnological Applications in Aquatic Health Management
3.1 Introduction
3.2 Nanomaterials Against Abiotic Stressors
3.2.1 Phosphate and Ammonia
3.2.2 Heavy Metals
Foreword
Preface
Acknowledgements
Contents
Editor and Contributors
1: Recent Developments in Biosensor Technology for Fisheries and Aquaculture
1.1 Introduction
1.2 Working Principle of Biosensor
1.3 Main Components of Biosensor
1.4 Types of Biosensors
1.4.1 Electrochemical Biosensor
1.4.1.1 Amperometric Biosensor
1.4.1.2 Potentiometric Biosensor
1.4.1.3 Impedimetric Biosensor
1.4.1.4 Voltammetric Biosensor
1.4.2 Physical Biosensor
1.4.2.1 Piezoelectric Biosensors
1.4.2.2 Thermometric Biosensor
1.4.3 Optical Biosensor
1.4.4 Wearable Biosensors
1.4.5 Enzyme Biosensor
1.4.6 DNA Biosensor
1.4.7 Immunosensors
1.4.7.1 Magnetic Biosensors
1.4.7.2 Resonant Biosensors
1.4.7.3 Thermal Detection Biosensor
1.4.8 Biosensors in Aquaculture
1.4.8.1 Ammonium Detection in Aquaculture
1.4.8.2 Detection of Water Pollutants
1.4.9 Biosensor for Fish Health Monitoring
1.4.9.1 Biosensor for VHSV Detection
1.4.9.2 Biosensor for Aphanomyces invadans
1.4.9.3 Aptamer-Based Biosensors for Pathogen Detection
1.4.9.4 Vibrio parahemolyticus
1.4.9.5 Vibrio vulnificus
1.4.9.6 Vibrio alginolyticus
1.4.9.7 Vibrio harveyi
1.4.9.8 Listeria monocytogenes
1.5 Conclusion
References
2: Application of CRISPR-Cas9 Technology in Fish
2.1 Introduction
2.2 Genome Editing Platforms
2.2.1 Zinc Finger Nuclease
2.2.2 Transcription Activator-Like Effector Nuclease
2.2.3 Clustered Regularly Interspersed Short Palindromic Sequence
2.2.3.1 Classification of CRISPR/Cas System
2.3 Genome Editing Using CRISPR/Cas9 System
2.3.1 Selection of Target Gene
2.3.2 Designing of gRNA
2.3.3 Delivery of sgRNA Complex
2.3.4 Production and Maintenance of Homozygous Mutant
2.4 Application of CRISPR/Cas9 in Fisheries and Aquaculture
2.4.1 Improvement of Aquaculture Important Traits
2.4.1.1 Somatic Growth and Production
2.4.2 Disease Resistance and Stress Tolerance
2.4.3 Sterility and Reproductive Confinement
2.4.4 Flesh Quality and Omega-3 Metabolism
2.4.5 Pigmentation
2.4.6 Application in Disease Diagnosis
2.4.7 Application in Functional Characterisation of Genes
2.4.8 Other Aquaculture Important Applications
2.5 Challenges in Application of GE-Based Tools in Fisheries and Aquaculture
2.5.1 Lack of Robust Knowledge of Teleost Genomes
2.5.2 Non-availability of Suitable Cell Lines
2.5.3 Development of Standardised Species-Specific Method
2.5.4 Effect of Whole Genome Duplication
2.5.5 Public Acceptance and Regulations
2.6 Commercialisation
2.7 Future Aspects and Conclusion
References
3: Nanotechnological Applications in Aquatic Health Management
3.1 Introduction
3.2 Nanomaterials Against Abiotic Stressors
3.2.1 Phosphate and Ammonia
3.2.2 Heavy Metals