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Title
Recombinant enzymes-- from basic science to commercialization [electronic resource] / Azura Amid, editor.
ISBN
9783319123974 electronic book
3319123971 electronic book
9783319123967
Published
Cham : Springer, 2015.
Language
English
Description
1 online resource (x, 188 pages) : illustrations
1 online resource.
Other Standard Identifiers
10.1007/978-3-319-12397-4 doi
Call Number
TP248.E5 R43 2015eb
Dewey Decimal Classification
572/.7
Summary
This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product.℗ℓ Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host.℗ℓ A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production.℗ℓ This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization.℗ℓ
Note
Includes index.
Access Note
Access limited to authorized users.
Source of Description
Online resource; title from PDF title page (SpringerLink, viewed January 26, 2015).
Available in Other Form
Print version: 9783319123967
Introduction to Recombinant Enzyme Pre-commercialization
Recombinant Enzyme: Cloning and Expression
Common Laboratory Procedure
Characterization of Recombinant Enzymes
Purification of Recombinant Protein for Industrial Use
Recombinant Enzyme Fermentation
Scaling-up Recombinant Enzyme Fermentation
Downstream Processing of Recombinant Enzymes for Commercialization
Economic and Environmental Evaluation of Recombinant Enzyme Production
Case Study: Recombinant Bromelain Selection
Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes
Case Study: Recombinant Bromelain Downstream Processing.
