Chapter 1 Introduction to Pathogenic Escherichia coli
1.1 Pathotypes of Escherichia coli causing diarrhoeal diseases
1.2 Escherichia coli
1.3 Pathogenic types of E. coli
1.4 Shiga toxin-producing and Enterohemorrhagic E. coli
1.4.1 Virulence determinants of Shiga-toxin producing E. coli
1.4.2 Shiga-toxin producing E. coli-mediated disease
1.4.3 Epidemiology of Shiga toxin-producing E. coli
1.4.4 Ecology of Shiga-toxin producing E. coli
1.4.5 Transmission of Shiga-toxin producing E. coli
1.4.6 Shiga-toxin producing E. coli occurrence in foods
1.5 Enterotoxigenic E. coli
1.6 Enteropathogenic E. coli
1.7 Enteroinvasive E. coli
1.8 Enteroaggregative and Diffusely Adherent E. coli..-1.9 References
Chapter 2 Isolation and detection of pathogenic Escherichia coli in foods
2.1 Introduction
2.2 General method of isolation for E. coli
2.3 Shiga toxin-producing E. coli
2.3.1 Culture and isolation of Shiga toxin-producing E. coli
2.3.2 Molecular detection of Shiga toxin-producing E. coli
2.4 Enumeration of Shiga toxin-producing E.coli
2.5 Immunological detection methods for pathogenic E. coli
2.6 Cell culture assays used for pathogenic E. coli
2.6.1 Cell cytotoxicity assay for Shiga toxin-producing E. coli
2.6.2 Cell adherence assays
2.7 Enteroinvasive E. coli
2.8 Enterotoxigenic E. coli
2.9 Enteropathogenic E. coli
2.10 Enteroaggregative E. coli and Diffusely Adherent E. coli
2.11 References
Chapter 3 Typing and Subtyping methods for pathogenic Escherichia coli
3.1 Introduction
3.2 Biochemical profiling
3.3 Serotyping
3.4 Phage typing
3.5 Multilocus enzyme electrophoresis
3.6 Multilocus sequence typing
3.7 Pulsed-field gel electrophoresis
3.8 Multiple-locus variable number tandem repeat analysis
3.9 Repetitive element palindromic polymerase chain reaction
3.10 Random amplified polymorphic DNA
3.11 Shiga toxin subtypes and bacteriophage insertion sites
3.12 Lineage specific polymorphism analysis
3.13 Whole genome sequencing
3.14
References
Chapter 4 Emerging and future trends and technologies for the detection and typing of Escherichia coli
4.1 Future trends in foodborne illness and E. coli
4.2 Future trends in the detection and typing of pathogens
4.2.1 Microarrays
4.2.2 Matrix Assisted Laser-Desorption Ionization-Time-Of-Flight Mass Spectrometry
4.2.3 Nanotechnology
4.2.4 Next-generating sequencing of the whole bacterial genome
4.3 References.