Preface; Contents; Part I: Overview; 1: Introduction; References; 2: Biomedical Significance and Development of "IVCT"; 2.1 Inevitable Problems with Conventional Preparation Techniques; 2.2 Quick-Freezing (QF) Method for Resected Fresh Tissues; 2.3 Development of In Vivo Cryotechnique (IVCT); References; 3: How to Perform "IVCT"; 3.1 Introduction; 3.2 Practical Procedures of In Vivo Cryoapparatus; 3.3 IVCT for Light Microscopy; 3.4 How to Prepare the Mixed Isopentane-Propane Cryogen; References; 4: Technical Merits with "IVCT"; 4.1 Tissue Depth of Well-Frozen Areas.

4.2 Plunging or Slamming Cryotechniques4.3 Thermal Conductance; References; Part II: Application of 'IVCT' to Various Mouse Organs; 5: Histochemical Analyses of Living Mouse Liver Under Different Hemodynamic Conditions; 5.1 Introduction; 5.2 Morphological Features of Living Mouse Liver; 5.3 Distribution of Serum Proteins and Glycogen; 5.4 Disrupted Cell Membrane Integrity; 5.5 Concluding Remarks; References; 6: Three-Dimensional Reconstruction of Liver Tissues with Confocal Laser Scanning Microscopy; 6.1 Introduction.

9: Detection of MAPK Signal Transduction Proteins in an Ischemia/Reperfusion Model of Small Intestines9.1 Introduction; 9.2 Histology and Immunolocalization of ERK1/2 and pERK1/2 in Normal Mouse Small Intestine by Various Preparative Methods; 9.3 ERK1/2 and pERK1/2 in 20 min Ischemia Group with IVCT-FS and IM-DH; 9.4 ERK1/2 and pERK1/2 in 20 min Ischemia with 60 min Reperfusion Group Prepared by IVCT-FS; 9.5 Concluding Remarks; References; 10: Histological Study and LYVE-1 Immunolocalization of Mesenteric Lymph Nodes; 10.1 Introduction.