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Supervisor's Foreword; Parts of the thesis have been published in the following journal articles:; Acknowledgements; Contents; 1 Introduction; Abstract; 1.1 Cell Membrane: Structure and Function; 1.1.1 Function of the Cell Membrane; 1.1.2 Composition of the Cell Membrane; 1.1.3 Structure of the Cell Membrane; 1.1.4 Phospholipid in Cell Membrane; 1.2 Membrane Proteins and in Vitro Reconstitution; 1.3 Membrane Binding Protein; 1.4 NMR Methods to Study Membrane Protein; 1.4.1 Background of NMR; 1.4.2 Use NMR to Study Protein Structure; 1.4.2.1 Protein Label and Sample Preparation.
1.4.2.2 Collecting Data and Chemical Shift Assignment1.4.2.3 Obtaining the Constraints for Structure Calculation; 1.4.2.4 Structure Calculation and Refinement; 1.4.3 Using NMR to Study Protein Function; 1.4.3.1 Protein Dynamics Study by NMR; 1.4.3.2 NMR Studies of Protein-Ligand Interactions; 1.4.3.3 Studies of the Membrane Binding Protein (MBP) by 31P NMR; References; 2 Lipid Molecular-Ion Interaction Study Based on Nanodisc; Abstract; 2.1 What Is a Nanodisc?; 2.2 Optimizing Nanodisc Assembly for NMR Studies; 2.2.1 Experiment Methodology and Materials; 2.2.2 Results.
3.2.7 Preparation of Nanodisc, Bicelles and Large Unilamellar Vesicles (LUVs)3.2.8 NMR Experiments; 3.2.9 Aromatic NOESY; 3.2.10 31P Spectrum; 3.3 Experiment Result; References; 4 Study on the Structure of Dengue Virus NS2B-NS3p in Complex with Aprotinin; Abstract; 4.1 Experiment Methods; 4.1.1 Clone, Expression and Purification of NS2B-NS3p; 4.1.2 NMR Sample Preparation; 4.1.3 NMR Spectra Collection and Data Processing; 4.2 Experiment Results; References; Conclusion and Future Work; Conclusion; Future Work; References.
1.4.2.2 Collecting Data and Chemical Shift Assignment1.4.2.3 Obtaining the Constraints for Structure Calculation; 1.4.2.4 Structure Calculation and Refinement; 1.4.3 Using NMR to Study Protein Function; 1.4.3.1 Protein Dynamics Study by NMR; 1.4.3.2 NMR Studies of Protein-Ligand Interactions; 1.4.3.3 Studies of the Membrane Binding Protein (MBP) by 31P NMR; References; 2 Lipid Molecular-Ion Interaction Study Based on Nanodisc; Abstract; 2.1 What Is a Nanodisc?; 2.2 Optimizing Nanodisc Assembly for NMR Studies; 2.2.1 Experiment Methodology and Materials; 2.2.2 Results.
3.2.7 Preparation of Nanodisc, Bicelles and Large Unilamellar Vesicles (LUVs)3.2.8 NMR Experiments; 3.2.9 Aromatic NOESY; 3.2.10 31P Spectrum; 3.3 Experiment Result; References; 4 Study on the Structure of Dengue Virus NS2B-NS3p in Complex with Aprotinin; Abstract; 4.1 Experiment Methods; 4.1.1 Clone, Expression and Purification of NS2B-NS3p; 4.1.2 NMR Sample Preparation; 4.1.3 NMR Spectra Collection and Data Processing; 4.2 Experiment Results; References; Conclusion and Future Work; Conclusion; Future Work; References.