Practical approach to mammalian cell and organ culture / edited by Tapan Kumar Mukherjee, Parth Malik, Srirupa Mukherjee.
Mukherjee, Tapan Kumar, editor.; Malik, Parth, editor.; Mukherjee, Srirupa, editor.
2022
QH585
Available Online

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Title
Practical approach to mammalian cell and organ culture / edited by Tapan Kumar Mukherjee, Parth Malik, Srirupa Mukherjee.
ISBN
9789811917318 (electronic bk.)
9811917310 (electronic bk.)
9789811917301 (print)
DOI
https://doi.org/10.1007/978-981-19-1731-8
Published
Singapore : Springer, 2022.
Language
English
Description
1 online resource : illustrations (some color)
Item Number
10.1007/978-981-19-1731-8 doi
Call Number
QH585
Dewey Decimal Classification
571.5/38
Summary
This Major Reference Work offers a detailed overview of culturing primary, secondary cell lines, tissues, and organs. It first introduces various types of mammalian cell cultures, infrastructure requirements for a mammalian cell-culture laboratory. The subsequent chapters present the detailed protocols for the isolation of mammalian hematologic organs and cells. It also discusses various cell-based assays for monitoring cell viability, cell proliferation, cytotoxicity, cell senescence, and cell death assays. In addition, the book addresses the various problems encountered while culturing animal cells, their possible causes, and suggested solutions, presenting detailed protocols for isolation and primary culturing of various mammalian cells and hematoimmunologic organs in two dimensions. Lastly, it reviews the various applications of animal-cell culture, stem-cell culture, and tissue and organ culture. As such, this reference book is highly relevant for students and professionals new to cell-culture work as well as to those wishing to expand their skills from cell-line cultures to primary cultures and from conventional 2D cultures to 3D cultures.
Access Note
Access limited to authorized users.
Source of Description
Title details screen.
Added Author
Mukherjee, Tapan Kumar, editor.
Malik, Parth, editor.
Mukherjee, Srirupa, editor.
Linked Resources
Online Access
Record Appears in
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All Resources
Mammalian Cell Culture: An Overview
Establishment of a Cell Culture Laboratory
Mammalian Cell Culture Laboratory: Equipment and Other Materials
Common Reagents and Medium for Mammalian Cell Culture
Microbial Contamination of Mammalian Cell Culture
Mammalian Cell Culture Types and Guidelines for Their Maintenance
Isolation and Purification of Various Mammalian Cells: Single Cell Isolation
Isolation and Primary Culture of Various Mammalian Cells
Primary Culture of Immunological Cells
Culture of Neuron and Glia Cells
Culture of Continuous Cell Lines
Stem Cell Culture and Its Applications
Mammalian Cell Culture in Three Dimensions: Basic Guidelines
Organ, Histotypic and Organotypic Culture, and Tissue Engineering
Large-Scale Culture of Mammalian Cells for Various Industrial Purposes
Experimental Mammalian Cell Culture-Based Assays
Mammalian Cells, Tissues and Organ Culture: Applications
Biosensors’ Utility in Mammalian Cell Culturing
Nanomaterials: Compatibility Towards Biological Interactions
Review on IPR and Technological Advancements in Nanotechnology for Nanomedicine
Emerging Drug Delivery Potential of Gold and Silver Nanoparticles to Lung and Breast Cancers
Mammalian Cells: Reliability as Model System in the Ecotoxicological Evaluation of Environmental Stressors
Bioimaging: Usefulness in Modern Day Research
Troubleshooting of Mammalian Cell Culture.

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