Transgenic ascidians / Yasunori Sasakura, editor.
Sasakura, Yasunori, editor.
2018
QH442.6
Available Online

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Title Transgenic ascidians / Yasunori Sasakura, editor.
ISBN 9789811075452 (electronic book)
981107545X (electronic book)
9789811075445
9811075441
DOI https://doi.org/10.1007/978-981-10-7545-2
Published Singapore, Singapore : Springer, [2018]
Language English
Description 1 online resource.
Item Number 10.1007/978-981-10-7545-2 doi
Call Number QH442.6
Dewey Decimal Classification 571.8
Summary This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.
Bibliography, etc. Note Includes bibliographical references and index.
Access Note Access limited to authorized users.
Digital File Characteristics text file PDF
Source of Description Online resource; title from PDF title page (SpringerLink, viewed March 21, 2018).
Added Author Sasakura, Yasunori, editor.
Series Advances in experimental medicine and biology ; v. 1029.
Available in Other Form Print version: 9789811075445
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Record Appears in Online Resources > Ebooks
All Resources
Introduction / Yasunori Sasakura
Microinjection of exogenous nucleic acids into eggs: Ciona species / Kenji Kobayashi, Yutaka Satou
Practical guide for ascidian microinjection: Phallusia mammillata / Hitoyoshi Yasuo, Alex McDougall
Microinjection of exogenous DNA into eggs of Halocynthia roretzi / Gaku Kumano
Electroporation in ascidians: history, theory and protocols / Robert W. Zeller
The use of cis-regulatory DNAs as molecular tools / Kotaro Shimai, Takehiro G. Kusakabe
Reporter analyses reveal redundant enhancers that confer robustness on cis-regulatory mechanisms / Shigeki Fujiwara, Cristian Cañestro
investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord / Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio
Purification of fluorescent labeled cells from dissociated Ciona embryos / Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen
Germline transgenesis in Ciona / Yasunori Sasakura
The enhancer trap in Ciona / Yasunori Sasakura
TALEN-based knockout system / Keita Yoshida, Nicholas Treen
CRISPR knockouts in Ciona embryos / Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi
Transgenic Techniques for Investigating Cell Biology During Development / Christina D. Cota
Cellular processes of notochord formation / William C. Smith
Morphology and physiology of the ascidian nervous systems and the effectors / Atsuo Nishino.

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